Anti-Phospho-Progesterone Receptor (Ser294) antibody (KLH)

Monoclonal

100 ul in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per ml BSA and 50% glycerol.

P06401

There is accumulating evidence to suggest that progesterone plays an essential role in the regulation of growth and differentiation of mammary glands and thus may play a key role in breast cancer (Edwards, 2005). The biological response to progesterone is mediated by two distinct forms of the human progesterone receptor (PR-A and PR-B forms). In most cell contexts, the B form functions as a transcriptional activator, whereas the A form functions as a transcriptional inhibitor of steroid hormones (Attia et al., 2000; Lin et al., 2003). Recently it has been demonstrated that there is differential hormone dependent regulation of the phosphorylation of the A and B forms of the receptor (Clemm et al., 2000). Treatment of T47D breast cancer cells with progestin agonist 190 294 increases the phosphorylation of Ser and Ser with different kinetics. These phosphorylation events may differentially affect the transcriptional activity of the receptor. Western blot of T47D cell lysate prepared from cells that had been incubated in the presence of the synthetic progestin agonist R5020 (500 nM) showing specific immunolabeling of

Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser294 conjugated to KLH.

Nucleus
Cytoplasm
Nucleoplasmic Shuttling Is Both Hormone-And Cell Cycle-Dependent
On Hormone Stimulation
Retained In The Cytoplasm In The G(1) And G(2)/M Phases
Isoform A: Nucleus
Mainly Nuclear
Isoform 4: Mitochondrion Outer Membrane

WB 1:1000; IHC 1:1000

Progesterone Receptor
Pr
Nuclear Receptor Subfamily 3 Group C Member 3

PRGR_HUMAN