ArtNr |
STJ120202-100 |
Hersteller |
St John's Laboratory
|
Menge |
100ul |
Kategorie |
|
Typ |
Antibody Monoclonal |
Applikationen |
WB, IHC |
Clon |
608 |
Specific against |
Human |
Host |
Mouse |
Isotype |
IgG1 |
ECLASS 10.1 |
42030590 |
ECLASS 11.0 |
42030590 |
UNSPSC |
12352203 |
Alias |
Anti-Progesterone Receptor antibody</br>Anti-Pr antibody</br>Anti-Nuclear Receptor Subfamily 3 Group C Member 3 antibody</br>Anti-PGR NR3C3 antibody |
Similar products |
Anti-Progesterone Receptor antibodyAnti-Pr antibodyAnti-Nuclear Receptor Subfamily 3 Group C Member 3 antibodyAnti-PGR NR3C3 antibody |
Lieferbar |
|
Clonality |
Monoclonal |
Conjugation |
KLH |
Formulation |
100 ul in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per ml BSA and 50% glycerol. |
Purification |
This antibody was protein g purified culture from supernatant. |
Uniprot ID |
P06401 |
Storage |
Store at-20° C, and avoid repeated freeze-thaw cycles. |
Description |
There is accumulating evidence to suggest that progesterone plays an essential role in the regulation of growth and differentiation of mammary glands and thus may play a key role in breast cancer (Edwards, 2005). The biological response to progesterone is mediated by two distinct forms of the human progesterone receptor (PR-A and PR-B forms). In most cell contexts, the B form functions as a transcriptional activator, whereas the A form functions as a transcriptional inhibitor of steroid hormones (Attia et al., 2000; Lin et al., 2003). Recently it has been demonstrated that there is differential hormone dependent regulation of the phosphorylation of the A and B forms of the receptor (Clemm et al., 2000). Treatment of T47D breast cancer cells with progestin agonist 190 294 increases the phosphorylation of Ser and Ser with different kinetics. These phosphorylation events may differentially affect the transcriptional activity of the receptor. Western blot of T47D cell lysate prepared from cells that had been incubated in the presence of the synthetic progestin agonist R5020 (500 nM) showing specific immunolabeling of |
Gene ID |
|
Immunogen |
Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser294 conjugated to KLH. |
Tissue specificity |
In reproductive tissues the expression of isoform A and isoform B varies as a consequence of developmental and hormonal status. Isoform A and isoform B are expressed in comparable levels in uterine glandular epithelium during the proliferative phase of the menstrual cycle. Expression of isoform B but not of isoform A persists in the glands during mid-secretory phase. In the stroma, isoform A is the predominant form throughout the cycle. Heterogeneous isoform expression between the glands of the endometrium basalis and functionalis is implying region-specific responses to hormonal stimuli. |
Cellular localization |
NucleusCytoplasmNucleoplasmic Shuttling Is Both Hormone-And Cell Cycle-DependentOn Hormone StimulationRetained In The Cytoplasm In The G(1) And G(2)/M PhasesIsoform A: NucleusMainly NuclearIsoform 4: Mitochondrion Outer Membrane |
Molecular weight |
90/120 kDa |
Dilution range |
WB 1:1000; IHC 1:1000 |
Alternative names |
Anti-Progesterone Receptor antibodyAnti-Pr antibodyAnti-Nuclear Receptor Subfamily 3 Group C Member 3 antibodyAnti-PGR NR3C3 antibody |
Protein name |
Progesterone ReceptorPrNuclear Receptor Subfamily 3 Group C Member 3 |
Note |
FOR RESEARCH USE ONLY (RUO). |
Swiss port key |
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