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Mouse SCF / C-kit ligand Protein (His Tag)

Mouse SCF / C-kit ligand Protein (His Tag)

ArtNr	50487-M08B-2
Hersteller	Sino Biological
Menge	2mg
Kategorie	Oncology By Cell Type Blastoma Carcinoma Glioma Hematology Leukemia Lymphoma Myeloma Melanoma Sarcoma Peptides & Proteins Recombinant Proteins
Typ	Proteins
Specific against	Mouse
ECLASS 10.1	32160409
ECLASS 11.0	32160409
UNSPSC	12352202
Alias	50487-M08B
Similar products	KITLG
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Molecule	KITLG
Storage	Store it under sterile conditions at -20C to -80C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Shipping	In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Protein Construction	A DNA sequence encoding the amino acids (Met 1-Ala 189) of mouse KITL (P20826-1) was fused with a polyhistidine tag at the C-terminus.
Expressed Host	Baculovirus-Insect Cells
Purity	> 95 % as determined by SDS-PAGE
Bio-Activity	1. Measured by its binding ability in a functional ELISA . Immobilized mouse KITL at 2 ug/ml (100 ul/well) can bind mouse CD117 with a linear ranger of 1.28-32 ng/ml . 2. Measured in a cell proliferation assay using TF-1 human erythroleukemic cells (Kitamura, T. et al. 1989, J. Cell Physiol. 140:323.) . The EC50 for this effect is typically 0.5-5 ng/ml. 3. Measured by its ability to inhibit Epo-dependent proliferation of TF1 human erythroleukemic cells. The ED50 for this effect is 6-24 ng/mL.
Endotoxin	< 1.0 EU per ug of the protein as determined by the LAL method
Predicted N Terminal	Lys 26
Molecule Mass	The secreted recombinant mouse KITL consists of 175 amino acids and has a calculated molecular mass of 19.7 kDa. The recombinant protein migrates as an approximately 23 kDa band in SDS-PAGE under reducing conditions.
Formulation	Lyophilized from sterile 20mM Tris, 50mM NaCl, pH 7.4
Accession	P20826-1
Amino acid sequence	Met1-Ala189
TAG	C-His

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

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50487-M08B-2-datasheet.pdf

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