Bio-Activity |
Measured by its ability to inhibit trypsin cleavage of a fluorogenic peptide substrate, Mca-RPKPVE-Nval-WRK(Dnp)-NH2 (Anaspec, Catalog#27114)
. The IC50 value is < 0.4 nM, as measured in 100uL reaction mixture containing 1.25 ng trypsin (Sigma, Catalog#T4799), 10 uM substrate, 50 mM Tris, 10 mM CaCl2, 0.15 M NaCl, 0.05% Brij-35, pH 7.5
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