ArtNr |
10204-H08H-100 |
Hersteller |
Sino Biological
|
Menge |
100ug |
Kategorie |
|
Typ |
Proteins |
Format |
Human Cell |
Specific against |
Human |
Host |
Human |
ECLASS 10.1 |
32160409 |
ECLASS 11.0 |
32160409 |
UNSPSC |
12352202 |
Alias |
Arc-1, CD324, CDHE, E-cad, E-Cadherin, ECAD, LCAM, UVO |
Similar products |
CDH1 |
Lieferbar |
|
Molecule |
CDH1 |
Synonym |
Arc-1, CD324, CDHE, E-cad, E-Cadherin, ECAD, LCAM, UVO |
Storage |
Store it under sterile conditions at -20C to -80C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
Shipping |
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. |
Protein Construction |
A DNA sequence encoding the extracellular domain of human E-Cad precusor (NP_004351.1) (Met 1-Ile 707) was fused with a C-terminal polyhistidine tag. |
Expressed Host |
Human Cells |
Purity |
> 95 % as determined by SDS-PAGE |
Bio-Activity |
Measured by the ability of the immobilized protein to support the adhesion of MCF-7 human breast adenocarcinoma cells. When cells are added to E-Cad coated plates (5 ug/mL, 100 uL/well), approximately 20%-40% of cells will adhere specifically after 90 minutes at 37 C. |
Endotoxin |
< 1.0 EU per ug of the protein as determined by the LAL method |
Predicted N Terminal |
Gln 23 & Asp 155 |
Molecule Mass |
The recombinant pro form of human E-Cad consists of 696 amino acids and has a calculated molecular mass of 77 kDa. As a result of glycosylation, the apparent molecular mass of rh E-Cad is approximately 80&95 kDa corresponding to the mature form and pro form respectively in SDS-PAGE under reducing conditions. |
Formulation |
Lyophilized from sterile PBS, pH 7.4 |
Accession |
NP_004351.1 |
Amino acid sequence |
Met1-Ile707 |
TAG |
C-His |
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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.