ArtNr |
10107-H08H-100 |
Hersteller |
Sino Biological
|
Menge |
100ug |
Kategorie |
|
Typ |
Proteins |
Specific against |
Human |
ECLASS 10.1 |
32160409 |
ECLASS 11.0 |
32160409 |
UNSPSC |
12352202 |
Alias |
CAIX, Carbonic Anhydrase IX, MN |
Lieferbar |
|
Molecule |
CA9 |
Synonym |
CAIX, Carbonic Anhydrase IX, MN |
Storage |
Store it under sterile conditions at -20C to -80C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
Shipping |
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. |
Protein Construction |
A DNA sequence encoding the human carbonic anhydrase IX (CA9) precursor (NP_001207.2) (Met 1-Asp 414) was expressed with a polyhistidine tag at the C-terminus. |
Expressed Host |
Human Cells |
Purity |
> 95 % as determined by SDS-PAGE |
Bio-Activity |
Measured by its esterase activity. The specific activity is >30 pmoles/min/ug, as measured with 1 mM 4-Nitrophenyl acetate and 2.5 ug enzyme at 400 nm in 100 uL of 12.5 mM Tris, 75 mM NaCl, pH 7.5. |
Endotoxin |
< 1.0 EU per ug of the protein as determined by the LAL method |
Predicted N Terminal |
Gln 38 |
Molecule Mass |
The recombinant human CA9 consists of 388 amino acids and predicts a molecular mass of 42.5 kDa. As a result of glycosylation, rhCA9 migrates with apparent molecular mass of 48 kDa in SDS-PAGE under reducing conditions. |
Formulation |
Lyophilized from sterile PBS, pH 7.4 |
Accession |
NP_001207.2 |
Amino acid sequence |
Met1-Asp414 |
TAG |
C-His |
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