ArtNr |
13044-H07BL-300 |
Hersteller |
Sino Biological
|
Menge |
300ug |
Kategorie |
|
Typ |
Cell Lysate |
Format |
Baculovirus-Insect cells |
Specific against |
Human |
Host |
Baculovirus-Infected Insect Cells |
ECLASS 10.1 |
32160890 |
ECLASS 11.0 |
32160890 |
UNSPSC |
41105330 |
Alias |
13044-H07BL |
Similar products |
TERF1 |
Lieferbar |
|
Molecule |
TERF1 |
Product Description |
Baculovirus-Insect Cells transfected lysate in which Human TERF1 / TRF1 has been over-expressed. The whole cell lysate is provided in 1X Sample Buffer. |
Expressed Host |
Baculovirus-Insect cells |
Preparation Method |
Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined with Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. |
Lysis Buffer |
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF |
Quality Control Testing |
12.5% SDS-PAGE Stained with Coomassie Blue |
Recommend Usage |
1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2. Re-dissolve the pellet using 200uL pure water and boiled for 2-5 min. 3. Store it at -80C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles. Notes: The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating. |
Storage Buffer |
In modified RIPA Lysis Buffer |
Storage Instruction |
Store at -80C. Aliquot to avoid repeated freezing and thawing |
Stability |
Samples are stable for up to twelve months from date of receipt at -80C |
Application notes |
WB: Use at an assay dependent dilution. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user. |
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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.