Human Alpha-fetoprotein Matched ELISA Antibody Pair Set

ELISA

Capture Antibody: Aliquot and store at -20C to -80C for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.

Detection Antibody: Protect it from prolonged exposure to light. Aliquot and store at -20C to -80C and for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.

Standard: Store lyophilized standard at -20C to -80C for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80C for up to 1 month. Avoid repeated freeze-thaw cycles.

0.1 mg/mL of mouse anti-AFP monoclonal antibody. Dilute to a working concentration of 2 ug/mL in CBS before coating.

Each vial contains 38 ng of recombinant AFP. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20C to -80C in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 0.7 ng/mL is recommended.

The Human AFP / alpha-fetoprotein ELISA Pair Set is for the quantitative determination of Human AFP / alpha-fetoprotein.
This ELISA Pair Set contains the basic components required for the development of sandwich ELISAs.
The Sino Biological ELISA Pair Set is a solid phase sandwich ELISA (Enzyme-Linked Immunosorbent Assay). It utilizes a monoclonal antibody specific for AFP / alpha-fetoprotein coated on a 96-well plate. Standards and samples are added to the wells, and any AFP / alpha-fetoprotein present binds to the immobilized antibody. The wells are washed and a horseradish peroxidase conjugated mouse anti-AFP / alpha-fetoprotein monoclonal antibody is then added, producing an antibody-antigen-antibody "sandwich". The wells are again washed and TMB substrate solution is loaded, which produces color in proportion to the amount of AFP / alpha-fetoprotein present in the sample. To end the enzyme reaction, the stop solution is added and absorbances of the microwell are read at 450 nm.