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Human Factor IX Matched ELISA Antibody Pair Set Europäischer Partner

ArtNr SEK11503-5
Hersteller Sino Biological
Menge 5 Plates
Quantity options 15 Plates 5 Plates
Kategorie
Typ ELISA Pair Set
Applikationen ELISA
Specific against Human (Homo sapiens)
ECLASS 10.1 32160605
ECLASS 11.0 32160605
UNSPSC 41116126
Alias SEK11503
Similar products F9
Lieferbar
Manufacturer - Category
ELISA
Molecule
F9
Storage
Capture Antibody: Aliquot and store at -20C to -80C for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.

Detection Antibody: Protect it from prolonged exposure to light. Aliquot and store at -20C to -80C and for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.

Standard: Store lyophilized standard at -20C to -80C for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80C for up to 1 month. Avoid repeated freeze-thaw cycles.
Conjugate
HRP
Capture Ab
1 mg/mL of rabbit anti-Human Coagulation Factor IX / FIX / F9 monoclonal antibody (in PBS, pH 7.4). Dilute to a working concentration of 2 ug/mL in PBS before coating.
Detection Ab
0.5 mg/mL of rabbit anti-Human Coagulation Factor IX / FIX / F9 monoclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4). Dilute to working concentration of 0.8 ug/mL in detection antibody dilution buffer before use.
Standard
Each vial contains 147 ng of recombinant Human Coagulation Factor IX / FIX / F9. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20C to -80C in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 7000 pg/mL is recommended.
Sensitivity
The minimum detectable dose of Human Coagulation Factor IX / FIX / F9 was determined to be approximately 109.38 pg/ml. This is defined as at least three times standard deviations above the mean optical density of 10 replicates of the zero standard.
Principle of the test
The Human Coagulation Factor IX / FIX / F9 ELISA Pair Set is for the quantitative determination of Human Coagulation Factor IX / FIX / F9.
The Sino Biological ELISA Pair Set is a solid phase sandwich ELISA (Enzyme-Linked Immunosorbent Assay). It utilizes a monoclonal antibody specific for Human Coagulation Factor IX / FIX / F9 coated on a 96-well plate. Standards and samples are added to the wells, and any Human Coagulation Factor IX / FIX / F9 present binds to the immobilized antibody. The wells are washed and a horseradish peroxidase conjugated rabbit anti-Human Coagulation Factor IX / FIX / F9 monoclonal antibody is then added, producing an antibody-antigen-antibody "sandwich". The wells are again washed and TMB substrate solution is loaded, which produces color in proportion to the amount of Human Coagulation Factor IX / FIX / F9 present in the sample. To end the enzyme reaction, the stop solution is added and absorbances of the microwell are read at 450 nm.

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 5 Plates
Lieferbar: In stock
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