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Human CD97 Matched ELISA Antibody Pair Set Europäischer Partner

ArtNr SEK11280-15
Hersteller Sino Biological
Menge 15 Plates
Quantity options 15 Plates 5 Plates
Kategorie
Typ ELISA Pair Set
Applikationen ELISA
Specific against Human (Homo sapiens)
ECLASS 10.1 32160605
ECLASS 11.0 32160605
UNSPSC 41116126
Alias SEK11280
Similar products CD97
Lieferbar
Manufacturer - Category
ELISA
Molecule
ADGRE5
Storage
Capture Antibody: Aliquot and store at -20C to -80C for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.

Detection Antibody: Protect it from prolonged exposure to light. Aliquot and store at -20C to -80C and for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.

Standard: Store lyophilized standard at -20C to -80C for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80C for up to 1 month. Avoid repeated freeze-thaw cycles.
Conjugate
HRP
Capture Ab
0.5 mg/mL of mouse anti-CD97 monoclonal antibody. Dilute to a working concentration of 2.0 ug/mL in CBS before coating.
Detection Ab
0.5 mg/mL mouse anti-CD97 monoclonal antibody conjugated to horseradish-peroxidase (HRP). Dilute to working concentration of 1.0 ug/mL in detection antibody dilution buffer before use
Standard
Each vial contains 80 ng of recombinant CD97. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20C to -80C in a manual defrost freezer. A seven-point standard curve usi ng 2-fold serial dilutions in sample dilution buffer, and a high standard of 2000 pg/mL is recommended.
Sensitivity
The minimum detectable dose of human CD97 was determined to be approximately 31.25 pg/ml. This is defined as at least three times standard deviations above the mean optical density of 10 replicates of the zero standard.
Principle of the test
The human CD97 ELISA Pair Set is for the quantitative determination of human CD97.
This ELISA Pair Set contains the basic components required for the development of sandwich ELISAs.
The Sino Biological ELISA Pair Set is a solid phase sandwich ELISA (Enzyme-Linked Immunosorbent Assay). It utilizes a monoclonal antibody specific for CD97coated on a 96-well plate. Standards and samples are added to the wells, andany CD97 present binds to the immobilized antibody. The wells are washed and ahorseradish peroxidase conjugated mouse anti-CD97 monoclonal antibody is thenadded, producing an antibody-antigen-antibody "sandwich". The wells are againwashed and TMB substrate solution is loaded, which produces color in proportionto the amount of CD97 present in the sample. To end the enzyme reaction, thestop solution is added and absorbances of the microwell are read at 450 nm.

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 15 Plates
Lieferbar: In stock
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