Human CD200 Matched ELISA Antibody Pair Set

ELISA

Capture Antibody: Aliquot and store at -20C to -80C for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.

Detection Antibody: Store at 4C and protect it from prolonged exposure to light for up to 6 months from date of receipt. DO NOT FREEZE!

Standard: Store lyophilized standard at -20C to -80C for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80C for up to 1 month. Avoid repeated freeze-thaw cycles.

0.5 mg/mL of Rabbit anti-CD200 monoclonal antibody (in PBS, pH 7.4). Dilute to a working concentration of 1 ug/mL in PBS before coating.

Each vial contains 190 ng of recombinant CD200. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20C to -80C in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 2 ng/mL is recommended.

The human CD200 ELISA Pair Set is for the quantitative determinationof human CD200.
This ELISA Pair Set contains the basic components required for the development of sandwich ELISAs.
The Sino Biological ELISA Pair Set is a solid phase sandwich ELISA (Enzyme-Linked Immunosorbent Assay). It utilizes a monoclonal antibody specific for CD200 coated on a 96-well plate. Standards and samples are added to the wells, and any CD200 present binds to the immobilized antibody. The wells are washed and a horseradish peroxidase conjugated rabbit anti-CD200 polyclonal antibody is then added, producing an antibody-antigen-antibody "sandwich". The wells are again washed and TMB substrate solution is loaded, which produces color in proportion to the amount of CD200 present in the sample. To end the enzyme reaction, the stop solution is added and absorbances of the microwell are read at 450 nm.