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Human CD157 Matched ELISA Antibody Pair Set

Human CD157 Matched ELISA Antibody Pair Set

ArtNr	SEK10060-5
Hersteller	Sino Biological
Menge	5 Plates
Quantity options	15 Plates 5 Plates
Kategorie	Antibodies ELISA Immunoassay Antibody Pairs
Typ	ELISA Pair Set
Applikationen	ELISA
Specific against	Human
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Alias	SEK10060
Similar products	BST1
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Manufacturer - Category	ELISA
Molecule	BST1
Storage	Capture Antibody: Aliquot and store at -20C to -80C for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles. Detection Antibody: Aliquot and store at -20C to -80C for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles. Standard: Store lyophilized standard at -20C to -80C for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80C for up to 1 month. Avoid repeated freeze-thaw cycles. Streptavidin-HRP: Store at 4C and protect it from prolonged exposure to light. DO NOT FREEZE! It is stable for up to 6 months from date of receipt.
Conjugate	Biotin
Capture Ab	0.5 mg/mL of mouse anti-BST1 monoclonal antibody. Dilute to a working concentration of 1 ug/mL in CBS before coating.
Detection Ab	0.3 mg/mL of biotinylated rabbit anti-BST1 polyclonal antibody. Dilute to a working concentration of 0.2 ug/mL in detection antibody dilution buffer before use.
Standard	Each vial contains 15 ng of recombinant BST1. Reconstitute with 1 mL detection antibody dilution buffer. A seven-point standard curve usi ng 2-fold serial dilutions in sample dilution buffer, and a high standard of 1 ng/mL is recommended.
Streptavidin-HRP	50 uL of streptavidin conjugated to horseradish-peroxidase. 1:2000 Dilution in detection antibody dilution buffer before use.
Sensitivity	The minimum detectable dose of human BST-1 / CD157 was determined to be approximately 15.6 pg/ml. This is defined as at least three times standard deviations above the mean optical density of 10 replicates of the zero standard.
Principle of the test	The human BST-1 / CD157 ELISA Pair Set is for the quantitative determination of human BST-1 / CD157. This ELISA Pair Set contains the basic components required for the development of sandwich ELISAs. The Sino Biological ELISA Pair Set is a solid phase sandwich ELISA (Enzyme-Linked Immunosorbent Assay). It utilizes a monoclonal antibody specific for BST-1 / CD157 coated on a 96-well plate. Standards and samples are added to the wells, and any BST-1 / CD157 present binds to the immobilized antibody. The wells are washed and a biotinylated rabbit anti-BST-1 / CD157 polyclonal antibody is then added, producing an antibody-antigen-antibody "sandwich". To produces color in proportion to the amount of BST-1 / CD157 present in the sample strepavidin-HRP and TMB substrate solution are loaded. The absorbances of the microwell are read at 450 nm.

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

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