MemGlow NR12A Membrane Polarity Probe ex 555 em 637 100-600 slides (MEMBRIGHT Family Probe)

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MemGlow NR12A is a solvatochromic photostable plasma membrane-targeting dye. Upon binding with a plasma membrane in a predominant liquid ordered (L_O) phase NR12A exhibits a 45-50 nm wavelength shift relative to liquid disordered (L_d) phase enabling investigators to examine the nanoscale distribution of local chemical polarity in plasma membranes. NR12A can also be used to label the plasma membrane for conventional fluorescence microscopy imaging.
NR12A Membrane Polarity Probe Product Functions (MG07)
The NR12A Probe is a long-chain probe with strong membrane binding
Suitable for Conventional Microscopy
Higher Affinity for Large and Giant Unilamelar Vesicles than traditional Nile Red
Exclusively Stain Plasma Membranes
Obtain high quality 3D Confocal Images of the Membrane Surface
For more detailed information on using this product see the About Tab
** 4 nmol of MemGlow will produce a 20uM stock solution when reconstituted with 200 ul of anhydrous DMSO

Figure 1. Diagram of NR12A's lipid order-dependent emission.

Figure 3. A) A 3D stacked image of KB cells stained with 20 nM NR12A (false colored orange). B) Widefield fluorescent imaging of live HeLa S1 cells labeled with 10 nM NR12A. HeLa S1 cells were imaged with a TRITC filter set, neutral density filter, a digital CCD camera, and 100x oil objective (false colored red).