Description |
CARD HyperOva (Japanese Patent No. 5927588) is an ultra-superovulation reagent that induces ovulation at higher efficiency when compared with the conventional superovulation induction method (PMSG-hCG method). Following the conventional method, we were only able to obtain approximately 20 ova per mouse (C57BL/6J) on average; using CARD HyperOva, however, we can obtain as many as 3-4 times more ova. CARDHyperOva F.D.is a hyperovulation reagent for use in reproductive engineering. It was developed at the Center for Animal Resources and Development (CARD), Kumamoto University. CARD HyperOva F.D. is freeze-dried to improve storage stability. |
Frequently Asked Questions |
1. Is the activity:dosage the same (0.1-0.2mL)? Yes, the activity of HyperOva FD is the same as the liquid version.
2. In the liquid version, there have been reports of a white precipitate (fibrin) in the tubes. Is this present in the FD version? No, fibrin was removed before lyophilization.
3. Can the HyperOva FD be reconstituted, aliquoted, and refrozen? If so, how long are the frozen aliquots able to be stored? Similar to standard HyperOva, after reconstitution HyperOva FD can be aliquoted and frozen ONE TIME without losing efficiency. Please note that there is no data yet regarding the shelf life of this reconstituted/frozen HyperOva FD.
4. Once reconstituted, how long can the HyperOva be stored in the fridge? No, once it is reconstituted, the HyperOva FD needs to either be used immediately or refrozen immediately (it can be refrozen one time for aliquoting). Do not store in the fridge for any amount of time. Please reconstitute at "room temperature"--generally 15-25 degrees C. |