JCSG Plus HT

1.0 mL of each 96 JCSG Plus HT in a 96 deep well polypropylene microplate.
The JCSG Plus is a general sparse matrix screen. 67 core reagents are based upon a statistical analysis performed by Page using results of the Joint Center for Structural Genomics (JCSG), in which a large number (473) of proteins from Thermotoga maritima were each set up against 480 reagents from 12 commercially available screens. 40 of the resulting 67 reagents (60%) are Hampton Research crystallization screen reagents (Crystal Screen, Crystal Screen 2, Crystal Screen Cryo, PEG/Ion Screen, Grid Screen Ammonium Sulfate, Grid Screen PEG 6000, Grid Screen PEG/LiCl, and Grid Screen MPD). The core 67 reagents are complemented with solutions from Index, an expansion of the JCSG selected by Newman to fill out the pH profile of the screen and to enhance the range of precipitants by including neutralized organic acids and Tacsimate. Therefore JCSG Plus is composed of 96 reagents, 69 (72%) of which are based on Hampton Research screens. If one wishes to screen beyond the reagents offered in JCSG Plus one should consider PEG/Ion or PEGRx for polymer biased reagents or SaltRx for salt biased reagents.
JCSG Plus contains 96 unique reagents, 10 ml each.
JCSG Plus HT contains 96 unique reagents in a single Deep Well block format.
Ready-to-use reagents are sterile filtered and formulated with ultra-pure Type 1 water, using the highest purity salts, polymers, organics and buffers. Individual reagents are available through the Hampton Research Custom Shop.