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Porcine IL-6 ELISA Kit

Porcine IL-6 ELISA Kit

ArtNr	ELP-IL6-2
Hersteller	Raybiotech
Menge	2 Plate Kit
Quantity options	1 Plate Kit 2 Plate Kit 5 Plate Kit
Kategorie	Oncology Metabolism Diabetes Glucose Homeostasis By Cell Type Carcinoma Hematology Leukemia Cell Biology Obesity Cell Culture Cytokines Cell Status Necrosis ELISA ELISA & Immunoassays ELISA Kits Immunoassay
Typ	Elisa-Kit
Specific against	Pig (Porcine, Sus scrofa domesticus)
Host	Hamster - Armenian
Sensitivity	45 pg/ml
Citations	1. Zhang K, Hou Q, Zhong Z, et al. Porcine reproductive and respiratory syndrome virus activates inflammasomes of porcine alveolar macrophages via its small envelope protein E. Virology. 2013 Aug 1,442(2):156–162. 2. Dorcaratto D, Burdio F, Fondevila D, et al. Radiofrequency is a secure and effective method for pancreatic transection in laparoscopic distal pancreatectomy: results of a randomized, controlled trial in an experimental model. Surg Endosc. 2013 Apr 13, doi:10.1007/s00464-013-2952-1 [Epub ahead of print] 3. Shimazu T., et al. Immunobiotic Lactobacillus jensenii Elicits Anti-Inflammatory Activity in Porcine Intestinal Epithelial Cells by Modulating Negative Regulators of the Toll-Like Receptor Signaling Pathway. Infect. Immun. 2012, 80(1):276. DOI: 10.1128/IAI.05729-11.
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Similar products	IL-6
Versandbedingung	Gekühlt
Lieferbar
Specificity	The sandwich ELISA antibody pair detects Porcine IL-6.
Manufacturer - Category	ELISA Kits\|Sandwich ELISA
Manufacturer - Targets	IL-6
Shipping Temperature	Blue ice
Storage Conditions	?-20°C
Description	RayBio® Porcine (pig) IL-6 ELISA Kit for cell culture supernatants, plasma, and serum samples. Lead time: Typically ships within 1-2 business days. No Friday shipments.
Compatible Sample Types	Cell Culture Supernatants, Plasma, Serum
Design Principle	Sandwich-based
Method of Detection	Colorimetric
Solid Support	96-well Microplate
Kit Components	Pre-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate
Other Materials Required	Distilled or deionized water Precision pipettes to deliver 2 ul to 1 ul volumes Adjustable 1-25 ul pipettes for reagent preparation 100 ul and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline	Prepare all reagents, samples and standards as instructed in the manual. Add 100 ul of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4C. Add 100 ul of prepared biotin antibody to each well. Incubate 1 h at RT. Add 100 ul of prepared Streptavidin solution to each well. Incubate 45 min at RT. Add 100 ul of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 ul of Stop Solution to each well. Read at 450 nm immediately.
Product Features	Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
Result Output	Quantitative
UNSPSC Code	41116158
Manufacturer - Specificity	The sandwich ELISA antibody pair detects Porcine IL-6.
Recommended Dilution	2 fold

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

ELP-IL6-2.pdf

ELP-IL6-2-datasheet.pdf

ELP-IL6-2-safety-datasheet.pdf