ArtNr |
ELP-IL6-1 |
Hersteller |
Raybiotech
|
Menge |
1 x 96-Well Strip Microplate Kit |
Kategorie |
|
Typ |
Elisa-Kit |
Specific against |
Porcine |
Host |
E.coli |
ECLASS 10.1 |
32160605 |
ECLASS 11.0 |
32160605 |
UNSPSC |
41116126 |
Similar products |
IL-6, IL-6 |
Lieferbar |
|
Quantity options |
|
Short description |
RayBio Porcine (pig) IL-6 ELISA Kit for serum, plasma, and cell culture supernatants |
Target Name and Synonyms |
IL-6 |
Solid Support |
96-well Microplate |
Design Principle |
Sandwich-based |
Result Output |
Quantitative |
Method of Detection |
Colorimetric |
Compatible Sample Types |
Cell Culture Supernatants, Plasma, Serum |
Sensitivity |
45 pg/ml |
Detection Range |
45 pg/ml - 10000 pg/ml |
Recommended Dilution (serum & plasma) |
2 fold |
Product Features |
- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
|
Kit Components |
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
|
Other Materials Required |
- Distilled or deionized water
- Precision pipettes to deliver 2 ul to 1 ul volumes
- Adjustable 1-25 ul pipettes for reagent preparation
- 100 ul and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
|
Protocol Summary |
- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 ul of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4C.
- Add 100 ul of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 ul of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 ul of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 ul of Stop Solution to each well.
- Read at 450 nm immediately.
|
Storage |
May be stored for up to 6 months at 2C to 8C from the date of shipment. Standard (recombinant protein) should be stored at -20C or -80C (recommended at -80C) after reconstitution. Opened Microplate Wells or reagents may be store for up to 1 month at 2C to 8C. Return unused wells to the pouch containing desiccant pack, reseal along entire edge. Note: the kit can be used within one year if the whole kit is stored at -20C. |
Citation |
1. Zhang K, Hou Q, Zhong Z, et al. Porcine reproductive and respiratory syndrome virus activates inflammasomes of porcine alveolar macrophages via its small envelope protein E. Virology. 2013 Aug 1, 442(2):156–162. 2. Dorcaratto D, Burdio F, Fondevila D, et al. Radiofrequency is a secure and effective method for pancreatic transection in laparoscopic distal pancreatectomy: results of a randomized, controlled trial in an experimental model. Surg Endosc. 2013 Apr 13, doi:10.1007/s00464-013-2952-1 [Epub ahead of print] 3. Shimazu T., et al. Immunobiotic Lactobacillus jensenii Elicits Anti-Inflammatory Activity in Porcine Intestinal Epithelial Cells by Modulating Negative Regulators of the Toll-Like Receptor Signaling Pathway. Infect. Immun. 2012, 80(1):276. DOI: 10.1128/IAI.05729-11. |
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