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Ovine IL-21 ELISA Kit

Ovine IL-21 ELISA Kit

ArtNr	ELO-IL21-5
Hersteller	Raybiotech
Menge	5 Plate Kit
Quantity options	1 Plate Kit 2 Plate Kit 5 Plate Kit
Kategorie	Cell Biology Cell Culture Cytokines ELISA ELISA & Immunoassays ELISA Kits Immunoassay
Typ	Elisa-Kit
Specific against	Sheep (Ovine, Ovis aries)
Host	Hamster - Armenian
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Alias	Interleukin
Versandbedingung	Gekühlt
Lieferbar
Species Detected	Ovine (sheep)
Design Principle	Sandwich-based
Method of Detection	Colorimetric
Quantitative/Semi-Quantitative	Quantitative
Solid Support	96-well Microplate
Gene Symbols	IL21
Kit Components	Pre- Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin- Conjugated H R P T M B One- Step Substrate
Other Materials Required	Distilled or deionized water Precision pipettes to deliver 2 Âµl to 1 Âµl volumes Adjustable 1-25 Âµl pipettes for reagent preparation100 Âµl and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for E L I S A data analysis
Protocol Outline	Prepare all reagents, samples and standards as instructed in the manual. Add 100 Âµl of standard or sample to each well. Incubate 2.5 h at R T or O/ N at 4Â° C. Add 100 Âµl of prepared biotin antibody to each well. Incubate 1 h at R T. Add 100 Âµl of prepared Streptavidin solution to each well. Incubate 45 min at R T. Add 100 Âµl of T M B One- Step Substrate Reagent to each well. Incubate 30 min at R T. Add 50 Âµl of Stop Solution to each well. Read at 450 nm immediately. Need your results faster? Try Ray Biotech's Speed E L I S A platform for quantitative detection in just three hours.
Protein Name & Synonyms	Interleukin
Additional Notes	Strip plates and additional reagents allow for use in multiple experimentsQuantitative protein detectionEstablishes normal rangeThe best products for confirmation of antibody array data
Note	This ELISA uses an antibody pair and standard that have been validated for multiplex array products, but not for the 96-well plate ELISA format. Thus, this ELISA requires a 4 - 7 week development phase, which RayBiotech begins after receiving an order for the kit. After development, the kit will have passed RayBiotech's ELISA quality control tests, and will thereafter have a typical 1 - 2 day lead time for orders placed.

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

ELO-IL21-5.pdf

ELO-IL21-5-datasheet.pdf

ELO-IL21-5-safety-datasheet.pdf