Vergleich

Ovine IL-21 ELISA Kit

ArtNr ELO-IL21-1
Hersteller Raybiotech
Menge 1 Plate Kit
Quantity options 1 Plate Kit 2 Plate Kit 5 Plate Kit
Kategorie
Typ Elisa-Kit
Specific against Sheep (Ovine, Ovis aries)
Host Hamster - Armenian
ECLASS 10.1 32160605
ECLASS 11.0 32160605
UNSPSC 41116126
Alias Interleukin
Versandbedingung Gekühlt
Lieferbar
Species Detected
Ovine (sheep)
Design Principle
Sandwich-based
Method of Detection
Colorimetric
Quantitative/Semi-Quantitative
Quantitative
Solid Support
96-well Microplate
Gene Symbols
IL21
Kit Components
Pre-
Coated 96-well Strip Microplate
Wash Buffer
Stop Solution
Assay Diluent(s)
Lyophilized Standard
Biotinylated Detection Antibody
Streptavidin-
Conjugated H
R
P
T
M
B One-
Step Substrate
Other Materials Required
Distilled or deionized water
Precision pipettes to deliver 2 µl to 1 µl volumes
Adjustable 1-25 µl pipettes for reagent preparation100 µl and 1 liter graduated cylinders
Tubes to prepare standard and sample dilutions
Absorbent paper
Microplate reader capable of measuring absorbance at 450nm
Log-log graph paper or computer and software for E
L
I
S
A data analysis
Protocol Outline
Prepare all reagents, samples and standards as instructed in the manual.
Add 100 µl of standard or sample to each well.
Incubate 2.5 h at R
T or O/
N at 4°
C.
Add 100 µl of prepared biotin antibody to each well.
Incubate 1 h at R
T.
Add 100 µl of prepared Streptavidin solution to each well.
Incubate 45 min at R
T.
Add 100 µl of T
M
B One-
Step Substrate Reagent to each well.
Incubate 30 min at R
T.
Add 50 µl of Stop Solution to each well.
Read at 450 nm immediately.
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Biotech's Speed
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L
I
S
A platform for quantitative detection in just three hours.
Protein Name & Synonyms
Interleukin
Additional Notes
Strip plates and additional reagents allow for use in multiple experimentsQuantitative protein detectionEstablishes normal rangeThe best products for confirmation of antibody array data
Note
This ELISA uses an antibody pair and standard that have been validated for multiplex array products, but not for the 96-well plate ELISA format. Thus, this ELISA requires a 4 - 7 week development phase, which RayBiotech begins after receiving an order for the kit. After development, the kit will have passed RayBiotech's ELISA quality control tests, and will thereafter have a typical 1 - 2 day lead time for orders placed.

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 1 Plate Kit
Lieferbar: In stock
lieferbar

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