ArtNr |
ELO-IL17A-5 |
Hersteller |
Raybiotech
|
Menge |
5 Plate Kit |
Quantity options |
1 Plate Kit
2 Plate Kit
5 Plate Kit
|
Kategorie |
|
Typ |
Elisa-Kit |
Specific against |
Sheep (Ovine, Ovis aries) |
Host |
Hamster - Armenian |
Sensitivity |
0.45 pg/ml |
ECLASS 10.1 |
32160605 |
ECLASS 11.0 |
32160605 |
UNSPSC |
41116126 |
Alias |
Interleukin-17A (IL-17) (IL-17A) |
Versandbedingung |
Gekühlt |
Lieferbar |
|
Specificity |
This ELISA kit shows no cross-reactivity with the following cytokines tested: Ovine CXCL9 (MIG), Decorin, IFN-gamma, IL-8 (CXCL8), IL-21, IP-10 (CXCL10), sFRP-3, TNF-alpha, VEGF-A. |
Species Detected |
Ovine (sheep) |
Compatible Sample Types |
Cell Culture Supernatants Plasma Serum |
Design Principle |
Sandwich-based |
Method of Detection |
Colorimetric |
Quantitative/Semi-Quantitative |
Quantitative |
Solid Support |
96-well Microplate |
Kit Components |
Pre- Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin- Conjugated H R P T M B One- Step Substrate |
Other Materials Required |
Distilled or deionized water Precision pipettes to deliver 2 µl to 1 µl volumes Adjustable 1-25 µl pipettes for reagent preparation100 µl and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for E L I S A data analysis |
Protocol Outline |
Prepare all reagents, samples and standards as instructed in the manual. Add 100 µl of standard or sample to each well. Incubate 2.5 h at R T or O/ N at 4° C. Add 100 µl of prepared biotin antibody to each well. Incubate 1 h at R T. Add 100 µl of prepared Streptavidin solution to each well. Incubate 45 min at R T. Add 100 µl of T M B One- Step Substrate Reagent to each well. Incubate 30 min at R T. Add 50 µl of Stop Solution to each well. Read at 450 nm immediately. |
Protein Name & Synonyms |
Interleukin-17A (IL-17) (IL-17A) |
Specicifity |
This ELISA kit shows no cross-reactivity with the following cytokines tested: Ovine CXCL9 (MIG), Decorin, IFN-gamma, IL-8 (CXCL8), IL-21, IP-10 (CXCL10), sFRP-3, TNF-alpha, VEGF-A. |
Recommended Dilution (serum & plasma) |
5 fold |
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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.