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Ovine IL-17A ELISA Kit

ArtNr ELO-IL17A-2
Hersteller Raybiotech
Menge 2 Plate Kit
Quantity options 1 Plate Kit 2 Plate Kit 5 Plate Kit
Kategorie
Typ Elisa-Kit
Specific against Sheep (Ovine, Ovis aries)
Host Hamster - Armenian
Sensitivity 0.45 pg/ml
ECLASS 10.1 32160605
ECLASS 11.0 32160605
UNSPSC 41116126
Alias Interleukin-17A (IL-17) (IL-17A)
Versandbedingung Gekühlt
Lieferbar
Specificity This ELISA kit shows no cross-reactivity with the following cytokines tested: Ovine CXCL9 (MIG), Decorin, IFN-gamma, IL-8 (CXCL8), IL-21, IP-10 (CXCL10), sFRP-3, TNF-alpha, VEGF-A.
Species Detected
Ovine (sheep)
Compatible Sample Types
Cell Culture Supernatants
Plasma
Serum
Design Principle
Sandwich-based
Method of Detection
Colorimetric
Quantitative/Semi-Quantitative
Quantitative
Solid Support
96-well Microplate
Kit Components
Pre-
Coated 96-well Strip Microplate
Wash Buffer
Stop Solution
Assay Diluent(s)
Lyophilized Standard
Biotinylated Detection Antibody
Streptavidin-
Conjugated H
R
P
T
M
B One-
Step Substrate
Other Materials Required
Distilled or deionized water
Precision pipettes to deliver 2 µl to 1 µl volumes
Adjustable 1-25 µl pipettes for reagent preparation100 µl and 1 liter graduated cylinders
Tubes to prepare standard and sample dilutions
Absorbent paper
Microplate reader capable of measuring absorbance at 450nm
Log-log graph paper or computer and software for E
L
I
S
A data analysis
Protocol Outline
Prepare all reagents, samples and standards as instructed in the manual.
Add 100 µl of standard or sample to each well.
Incubate 2.5 h at R
T or O/
N at 4°
C.
Add 100 µl of prepared biotin antibody to each well.
Incubate 1 h at R
T.
Add 100 µl of prepared Streptavidin solution to each well.
Incubate 45 min at R
T.
Add 100 µl of T
M
B One-
Step Substrate Reagent to each well.
Incubate 30 min at R
T.
Add 50 µl of Stop Solution to each well.
Read at 450 nm immediately.
Protein Name & Synonyms
Interleukin-17A (IL-17) (IL-17A)
Specicifity
This ELISA kit shows no cross-reactivity with the following cytokines tested: Ovine CXCL9 (MIG), Decorin, IFN-gamma, IL-8 (CXCL8), IL-21, IP-10 (CXCL10), sFRP-3, TNF-alpha, VEGF-A.
Recommended Dilution (serum & plasma)
5 fold

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 2 Plate Kit
Lieferbar: In stock
lieferbar

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