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Ovine IL-17A ELISA Kit

Ovine IL-17A ELISA Kit

ArtNr	ELO-IL17A-1
Hersteller	Raybiotech
Menge	1 Plate Kit
Quantity options	1 Plate Kit 2 Plate Kit 5 Plate Kit
Kategorie	Neuroscience Neuroinflammation Cell Biology Cell Culture Cytokines ELISA ELISA & Immunoassays ELISA Kits Immunoassay
Typ	Elisa-Kit
Specific against	Sheep (Ovine, Ovis aries)
Host	Hamster - Armenian
Sensitivity	0.45 pg/ml
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Alias	Interleukin-17A (IL-17) (IL-17A)
Versandbedingung	Gekühlt
Lieferbar
Specificity	This ELISA kit shows no cross-reactivity with the following cytokines tested: Ovine CXCL9 (MIG), Decorin, IFN-gamma, IL-8 (CXCL8), IL-21, IP-10 (CXCL10), sFRP-3, TNF-alpha, VEGF-A.
Species Detected	Ovine (sheep)
Compatible Sample Types	Cell Culture Supernatants Plasma Serum
Design Principle	Sandwich-based
Method of Detection	Colorimetric
Quantitative/Semi-Quantitative	Quantitative
Solid Support	96-well Microplate
Kit Components	Pre- Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin- Conjugated H R P T M B One- Step Substrate
Other Materials Required	Distilled or deionized water Precision pipettes to deliver 2 Âµl to 1 Âµl volumes Adjustable 1-25 Âµl pipettes for reagent preparation100 Âµl and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for E L I S A data analysis
Protocol Outline	Prepare all reagents, samples and standards as instructed in the manual. Add 100 Âµl of standard or sample to each well. Incubate 2.5 h at R T or O/ N at 4Â° C. Add 100 Âµl of prepared biotin antibody to each well. Incubate 1 h at R T. Add 100 Âµl of prepared Streptavidin solution to each well. Incubate 45 min at R T. Add 100 Âµl of T M B One- Step Substrate Reagent to each well. Incubate 30 min at R T. Add 50 Âµl of Stop Solution to each well. Read at 450 nm immediately.
Protein Name & Synonyms	Interleukin-17A (IL-17) (IL-17A)
Specicifity	This ELISA kit shows no cross-reactivity with the following cytokines tested: Ovine CXCL9 (MIG), Decorin, IFN-gamma, IL-8 (CXCL8), IL-21, IP-10 (CXCL10), sFRP-3, TNF-alpha, VEGF-A.
Recommended Dilution (serum & plasma)	5 fold

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

ELO-IL17A-1.pdf

ELO-IL17A-1-datasheet.pdf

ELO-IL17A-1-safety-datasheet.pdf