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Custom Human EGF Speed-E ELISA Kit

Custom Human EGF Speed-E ELISA Kit

ArtNr	ELHS-EGF-5
Hersteller	Raybiotech
Menge	5 Plate Kit
Quantity options	1 Plate Kit 2 Plate Kit 5 Plate Kit
Kategorie	ELISA ELISA & Immunoassays ELISA Kits Immunoassay
Typ	Elisa-Kit
Specific against	Human (Homo sapiens)
Host	Hamster - Armenian
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Versandbedingung	Gekühlt
Lieferbar
Design Principle	Sandwich-based
Method of Detection	Colorimetric
Quantitative/Semi-Quantitative	Quantitative
Solid Support	96-well Microplate
Gene Symbols	EGF
Kit Components	Streptavidin- Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Capture Antibody H R P Conjugated Detection Antibody T M B One- Step Substrate
Other Materials Required	Distilled or deionized water Precision pipettes to deliver 2 Âµl to 1 Âµl volumes Adjustable 1-25 Âµl pipettes for reagent preparation100 Âµl and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for E L I S A data analysis
Protocol Outline	Prepare all reagents, samples and standards as instructed in the manual. Add 70 Âµl of the Antibody Cocktail to each well. Incubate 10 minutes at R T. Add 50 Âµl of sample or standard to each well. Incubate 2 h at R T. Wash Wells Add 100 Âµl of T M B One- Step Substrate Reagent to each well. Incubate 30 min at R T. Add 50 Âµl of Stop Solution to each well. Read at 450 nm immediately.
Storage/Stability	May be stored for up to 6 months at 2Â° to 8°C from the date of shipment. Opened Microplate Wells or reagents may be store for up to 1 month at 2Â° to 8°C. Return unused wells to the pouch containing desiccant pack, reseal along entire edge. Reconstituted standard can be stored at -80°C for up to 1 week.Note: the kit can be used within one year if the whole kit is stored at -20°C. Avoid repeated freeze-thaw cycles.
Protein Name & Synonyms	Pro-epidermal growth factor (EGF) [Cleaved into: Epidermal growth factor (Urogastrone)]

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

ELHS-EGF-5.pdf

ELHS-EGF-5-datasheet.pdf

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Menge: 5 Plate Kit

lieferbar

Lieferung vsl. bis 24.01.2025

Express-Lieferung: 4 Tage sparen

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