ArtNr
ELM-bIGH3-2
Hersteller
Raybiotech
Menge
2 Plate Kit
Quantity options
1 Plate Kit
2 Plate Kit
5 Plate Kit
Kategorie
Typ
Elisa-Kit
Specific against
Mouse
Host
Hamster - Armenian
Sensitivity
3 pg/ml
ECLASS 10.1
32160605
ECLASS 11.0
32160605
UNSPSC
41116126
Similar products
beta IG-H3
Lieferbar
Specificity
This ELISA antibody pair detects mouse beta-IGH3. Other species not determined.
Quantity options
Compatible Sample Types
Cell Culture Supernatants, Plasma, Serum
Design Principle
Sandwich-based
Kit Components
Pre-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate
Method of Detection
Colorimetric
Other Materials Required
Distilled or deionized water Precision pipettes to deliver 2 ul to 1 ul volumes Adjustable 1-25 ul pipettes for reagent preparation 100 ul and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis
Product Features
Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
Protocol Summary
Prepare all reagents, samples and standards as instructed in the manual. Add 100 ul of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4C. Add 100 ul of prepared biotin antibody to each well. Incubate 1 h at RT. Add 100 ul of prepared Streptavidin solution to each well. Incubate 45 min at RT. Add 100 ul of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 ul of Stop Solution to each well. Read at 450 nm immediately.
Recommended Dilution (serum & plasma)
20 - 200 fold
Result Output
Quantitative
Short description
RayBio Mouse beta IG-H3 (BIGH3) ELISA Kit for serum, plasma, and cell culture supernatants
Solid Support
96-well Microplate
Storage
May be stored for up to 6 months at 2C to 8C from the date of shipment. Standard (recombinant protein) should be stored at -20C or -80C (recommended at -80C) after reconstitution. Opened Microplate Wells or reagents may be store for up to 1 month at 2C to 8C. Return unused wells to the pouch containing desiccant pack, reseal along entire edge. Note: the kit can be used within one year if the whole kit is stored at -20C.
Target Name and Synonyms
beta IG-H3
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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.