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Human IL-31 RA ELISA Kit

Human IL-31 RA ELISA Kit

ArtNr	ELH-IL31RA-1
Hersteller	Raybiotech
Menge	1 Plate Kit
Quantity options	1 Plate Kit 2 Plate Kit 5 Plate Kit
Kategorie	Oncology Neuroscience Neural Lineage Markers Cell Biology Cell Culture Cytokines ELISA Cardiovascular Research Atherosclerosis ELISA & Immunoassays ELISA Kits Immunoassay By Organ Skin Cancer Hepatic Cancer Glial Cell Markers
Typ	Elisa-Kit
Specific against	Human (Homo sapiens)
Host	Hamster - Armenian
Sensitivity	0.98 ng/ml
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Alias	Interleukin-31 receptor subunit alpha (IL-31 receptor subunit alpha) (IL-31R subunit alpha) (IL-31R-alpha) (IL-31RA) (Cytokine receptor-like 3) (GLM-R) (hGLM-R) (Gp130-like monocyte receptor) (Gp130-like receptor) (ZcytoR17)
Versandbedingung	Gekühlt
Lieferbar
Specificity	This ELISA antibody pair detects Human IL-31 RA. Other species not determined.
Compatible Sample Types	Cell Culture Supernatants Plasma Serum
Design Principle	Sandwich-based
Method of Detection	Colorimetric
Quantitative/Semi-Quantitative	Quantitative
Solid Support	96-well Microplate
Kit Components	Pre- Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin- Conjugated H R P T M B One- Step Substrate
Other Materials Required	Distilled or deionized water Precision pipettes to deliver 2 Âµl to 1 Âµl volumes Adjustable 1-25 Âµl pipettes for reagent preparation100 Âµl and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for E L I S A data analysis
Protocol Outline	Prepare all reagents, samples and standards as instructed in the manual. Add 100 Âµl of standard or sample to each well. Incubate 2.5 h at R T or O/ N at 4Â° C. Add 100 Âµl of prepared biotin antibody to each well. Incubate 1 h at R T. Add 100 Âµl of prepared Streptavidin solution to each well. Incubate 45 min at R T. Add 100 Âµl of T M B One- Step Substrate Reagent to each well. Incubate 30 min at R T. Add 50 Âµl of Stop Solution to each well. Read at 450 nm immediately.
Protein Name & Synonyms	Interleukin-31 receptor subunit alpha (IL-31 receptor subunit alpha) (IL-31R subunit alpha) (IL-31R-alpha) (IL-31RA) (Cytokine receptor-like 3) (GLM-R) (hGLM-R) (Gp130-like monocyte receptor) (Gp130-like receptor) (ZcytoR17)
Specicifity	This ELISA antibody pair detects Human IL-31 RA. Other species not determined.
Recommended Dilution (serum & plasma)	3 fold
Gene Names	CRL3GPLIL31RAUNQ6368/PRO21073/PRO21384

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