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Human IL-22 R alpha 1 ELISA Kit

ArtNr ELH-IL22Ra1-1
Hersteller Raybiotech
Menge 1 Plate Kit
Quantity options 1 Plate Kit 2 Plate Kit 5 Plate Kit
Kategorie
Typ Elisa-Kit
Specific against Human (Homo sapiens)
Host Hamster - Armenian
Sensitivity 10.5 pg/ml
ECLASS 10.1 32160605
ECLASS 11.0 32160605
UNSPSC 41116126
Alias Interleukin-22 receptor subunit alpha-1 (IL-22 receptor subunit alpha-1) (IL-22R-alpha-1) (IL-22RA1) (Cytokine receptor class-II member 9) (Cytokine receptor family 2 member 9) (CRF2-9) (ZcytoR11)
Versandbedingung Gekühlt
Lieferbar
Specificity This ELISA antibody pair recognizes Human IL-22 R alpha 1.
Compatible Sample Types
Cell Culture Supernatants
Plasma
Serum
Design Principle
Sandwich-based
Method of Detection
Colorimetric
Quantitative/Semi-Quantitative
Quantitative
Solid Support
96-well Microplate
Gene Symbols
IL22RIL22RA1
Kit Components
Pre-
Coated 96-well Strip Microplate
Wash Buffer
Stop Solution
Assay Diluent(s)
Lyophilized Standard
Biotinylated Detection Antibody
Streptavidin-
Conjugated H
R
P
T
M
B One-
Step Substrate
Other Materials Required
Distilled or deionized water
Precision pipettes to deliver 2 µl to 1 µl volumes
Adjustable 1-25 µl pipettes for reagent preparation100 µl and 1 liter graduated cylinders
Tubes to prepare standard and sample dilutions
Absorbent paper
Microplate reader capable of measuring absorbance at 450nm
Log-log graph paper or computer and software for E
L
I
S
A data analysis
Protocol Outline
Prepare all reagents, samples and standards as instructed in the manual.
Add 100 µl of standard or sample to each well.
Incubate 2.5 h at R
T or O/
N at 4°
C.
Add 100 µl of prepared biotin antibody to each well.
Incubate 1 h at R
T.
Add 100 µl of prepared Streptavidin solution to each well.
Incubate 45 min at R
T.
Add 100 µl of T
M
B One-
Step Substrate Reagent to each well.
Incubate 30 min at R
T.
Add 50 µl of Stop Solution to each well.
Read at 450 nm immediately.
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Biotech's Speed
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L
I
S
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Protein Name & Synonyms
Interleukin-22 receptor subunit alpha-1 (IL-22 receptor subunit alpha-1) (IL-22R-alpha-1) (IL-22RA1) (Cytokine receptor class-II member 9) (Cytokine receptor family 2 member 9) (CRF2-9) (ZcytoR11)
Specicifity
This ELISA antibody pair recognizes Human IL-22 R alpha 1.
Recommended Dilution (serum & plasma)
2 fold
Additional Notes
Strip plates and additional reagents allow for use in multiple experimentsQuantitative protein detectionEstablishes normal rangeThe best products for confirmation of antibody array data

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 1 Plate Kit
Lieferbar: In stock
lieferbar

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