ArtNr |
ELH-IL20Rb-2 |
Hersteller |
Raybiotech
|
Menge |
2 Plate Kit |
Quantity options |
1 Plate Kit
2 Plate Kit
5 Plate Kit
|
Kategorie |
|
Typ |
Elisa-Kit |
Specific against |
Human (Homo sapiens) |
Host |
Hamster - Armenian |
ECLASS 10.1 |
32160605 |
ECLASS 11.0 |
32160605 |
UNSPSC |
41116126 |
Alias |
Interleukin-20 receptor subunit beta (IL-20 receptor subunit beta) (IL-20R-beta) (IL-20RB) (Fibronectin type III domain containing 6) (FNDC6) (IL-20R2) |
Versandbedingung |
Gekühlt |
Lieferbar |
|
Specificity |
This ELISA antibody pair recognizes human IL-20 R beta. Other species not yet determined. |
Design Principle |
Sandwich-based |
Method of Detection |
Colorimetric |
Quantitative/Semi-Quantitative |
Quantitative |
Solid Support |
96-well Microplate |
Gene Symbols |
DIRS1IL20RBUNQ557/PRO1114 |
Kit Components |
Pre- Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin- Conjugated H R P T M B One- Step Substrate |
Other Materials Required |
Distilled or deionized water Precision pipettes to deliver 2 µl to 1 µl volumes Adjustable 1-25 µl pipettes for reagent preparation100 µl and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for E L I S A data analysis |
Protocol Outline |
Prepare all reagents, samples and standards as instructed in the manual. Add 100 µl of standard or sample to each well. Incubate 2.5 h at R T or O/ N at 4° C. Add 100 µl of prepared biotin antibody to each well. Incubate 1 h at R T. Add 100 µl of prepared Streptavidin solution to each well. Incubate 45 min at R T. Add 100 µl of T M B One- Step Substrate Reagent to each well. Incubate 30 min at R T. Add 50 µl of Stop Solution to each well. Read at 450 nm immediately. Need your results faster? Try Ray Biotech's Speed E L I S A platform for quantitative detection in just three hours. |
Protein Name & Synonyms |
Interleukin-20 receptor subunit beta (IL-20 receptor subunit beta) (IL-20R-beta) (IL-20RB) (Fibronectin type III domain containing 6) (FNDC6) (IL-20R2) |
Specicifity |
This ELISA antibody pair recognizes human IL-20 R beta. Other species not yet determined. |
Additional Notes |
Strip plates and additional reagents allow for use in multiple experimentsQuantitative protein detectionEstablishes normal rangeThe best products for confirmation of antibody array data |
Note |
This ELISA uses an antibody pair and standard that have been validated for multiplex array products, but not for the 96-well plate ELISA format. Thus, this ELISA requires a 4 - 7 week development phase, which RayBiotech begins after receiving an order for the kit. After development, the kit will have passed RayBiotech's ELISA quality control tests, and will thereafter have a typical 1 - 2 day lead time for orders placed. |
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