ArtNr |
PEL-ITK-Y-1 |
Hersteller |
Raybiotech
|
Menge |
1 Plate Kit |
Quantity options |
1 Plate Kit
2 Plate Kit
5 Plate Kit
|
Kategorie |
|
Typ |
Elisa-Kit |
Specific against |
Human |
Host |
Hamster - Armenian |
Sensitivity |
The antibody pair provided in this kit recognizes Human Tyrosine-Phosphorylated-ITK. |
ECLASS 10.1 |
32160605 |
ECLASS 11.0 |
32160605 |
UNSPSC |
41116126 |
Similar products |
ITK |
Lieferbar |
|
Specificity |
The antibody pair provided in this kit recognizes Human Tyrosine-Phosphorylated-ITK. |
Quantity options |
|
Compatible Sample Types |
Tissue Lysates, Cell Lysates |
Design Principle |
Sandwich-based |
Kit Components |
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Biotinylated Anti-Phosphotyrosine Antibody
- Stop Solution
- Assay Diluent(s)
- Positive Control Sample
- Lysis Buffer
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
|
Method of Detection |
Colorimetric |
Other Materials Required |
- Distilled or deionized water
- 100 ml and 1 liter graduated cylinders
- Tubes to prepare sample dilutions
- Protease and Phosphatase inhibitors
- Precision pipettes to deliver 2 µ, l to 1 ml volumes
- Adjustable 1-25 ml pipettes for reagent preparation
- Benchtop rocker or shaker
- Microplate reader capable of measuring absorbance at 450 nm
|
Product Features |
- Rapidly measure phosphorylated protein in lysates
- Screen numerous different cell lysates without performing a Western Blot analysis
- Minimal hands-on time, convenient, and non-radioactive material
|
Protocol Summary |
- Prepare all reagents and samples as instructed in the manual.
- Add 100 µ, l of sample or positive control to each well.
- Incubate 2.5 h at RT or O/N at 4 °, C.
- Add 100 µ, l of prepared primary antibody to each well.
- Incubate 1 h at RT.
- Add 100 µ, l of prepared 1X HRP-Streptavidin to each well.
- Incubate 1 h at RT.
- Add 100 µ, l of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µ, l of Stop Solution to each well.
- Read at 450 nm immediately.
|
Result Output |
Semi-Quantitative |
Short description |
RayBio Custom Human Phosphotyrosine ITK ELISA Kit (Available only on demand) |
Solid Support |
96-well Microplate |
Storage |
Upon receipt, the kit should be stored at -20 °, C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °, C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °, C. Reconstituted Positive Control (Item K) should be stored at -70 °, C. |
Target Name and Synonyms |
ITK (P-Tyr) |
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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.