Vergleich

Human TIMP-1 ELISA

Hersteller Raybiotech
Kategorie
Typ Elisa-Kit
Specific against Human
Menge 1 x 96-Well Strip Microplate Kit
Host Mouse
ArtNr ELH-TIMP1-1
Targets TIMP1
eClass 6.1 32160605
eClass 9.0 32160605
Lieferbar
Quantity options
Short description
RayBio Human TIMP-1 ELISA Kit for serum, plasma, cell culture supernatants, and urine
Target Name and Synonyms
TIMP-1
Solid Support
96-well Microplate
Design Principle
Sandwich-based
Result Output
Quantitative
Method of Detection
Colorimetric
Compatible Sample Types
Cell Culture Supernatants, Plasma, Serum, Urine
Sensitivity
40 pg/ml
Detection Range
2 pg/ml - 1200 pg/ml
Recommended Dilution (serum & plasma)
5-100 fold
Specificity
This ELISA kit shows no cross-reactivity with any of the cytokines tested (e.g., human ANG, CD23, Eotaxin, GCSF, GM-CSF, GRO- alpha, GRO-beta, GRO-gamma, I-309, IFN-gamma, IL-1alpha, IL-1beta, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-10, IL-12 (p40), IL-12 (p70), IL-15, IL-16, IP-10, MCP-1, MCP-2, MCP-3, MCP-4, MCSF, MIG, MIP-1alpha, MIP-1beta, NAP-2, PDGF, PF-4, PARC, SCF, SDF-1alpha, TNFbeta, TGFbeta1, TGFbeta2, TGFbeta3, VEGF).
Product Features
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data
Kit Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Other Materials Required
  • Distilled or deionized water
  • Precision pipettes to deliver 2 ul to 1 ul volumes
  • Adjustable 1-25 ul pipettes for reagent preparation
  • 100 ul and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
Protocol Summary
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 ul of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4C.
  4. Add 100 ul of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 ul of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 ul of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 ul of Stop Solution to each well.
  11. Read at 450 nm immediately.
Storage
May be stored for up to 6 months at 2C to 8C from the date of shipment. Standard (recombinant protein) should be stored at -20C or -80C (recommended at -80C) after reconstitution. Opened Microplate Wells or reagents may be store for up to 1 month at 2C to 8C. Return unused wells to the pouch containing desiccant pack, reseal along entire edge. Note: the kit can be used within one year if the whole kit is stored at -20C.
Citation
1. Steed D, Trumpower C, Duffy D, Smith C, Marshall V, et al. Amnion-derived Cellular Cytokine Solution A Physiological Combination of Cytokines for Wound Healing. Eplasty. 2008, 8:e18.
2. Hampel U, Klonisch T, Sel S, et al. Insulin-like Factor 3 Promotes Wound Healing at the Ocular Surface. Endocrinology. 2013, 154(6):2034–2045.
3. Kralisch S, Lossner U, Bluher M, Paschke R, Stumvoll M, Fasshauer M. Tissue inhibitor of metalloproteinase 1 expression and secretion are induced by beta-adrenergic stimulation in 3T3-L1 adipocytes. J Endocrinol. 2006, 189:665-670.
4. Upadhyay A, Sharma G, Kivivuori S, Raye W S, Zabihi E, et al. Role of a LIF antagonist in LIF and OSM induced MMP-1, MMP-3, and TIMP-1 expression by primary articular chondrocytes. Cytokine. 2009, 46:332–338.
5. Fernandez-Yunquera A., et al. Everolimus immunosuppression reduces the serum expression of fibrosis markers in liver transplant recipients. World J Transplant 2014, 4(2): 133-140. http://dx.doi.org/10.5500/wjt.v4.i2.133
6. Kilic Z., et al. Circulating matrix metalloproteinases and tissue inhibitors of metalloproteinases levels in pediatric patients with congenital heart disease: Relationship to cardiac functions. Anatolian J of Card. Published ahead of print, June 25th 2014. DOI:10.5152/akd.2014.4950
7. Esa S., et al. Study of the level of sputum matrix metalloproteinase-9 (MMP-9) and tissue inhibitor metalloproteinase-1 (TIMP-1) in COPD patients. Egyptian Journal of Chest Diseases and Tuberculosis Available online 18 July 2014 DOI: 10.1016/j.ejcdt.2014.06.014
8. Ghodpage P., et al. Influence of phase I periodontal therapy on levels of matrix metalloproteinase 1 and tissue inhibitor of metalloproteinase 1 . The Saudi Dental Journal. Available online 15 July 2014 DOI: 10.1016/j.sdentj.2014.05.003
9. Acar B., Nese, Z., et al. Serum MMP-2, MMP-9, TIMP-1 and TIMP-2 levels in multiple sclerosis clinical subtypes and their diagnostic value in the progressive disease course. Biomedical Research, Volume 25, Issue 3, Pages 343 - 350. 2014.
10. Gluba-Brzozka A., et al. Markers of increased cardiovascular risk in patients with chronic kidney disease. Lipids in Health and Disease 2014, 13:135 doi:10.1186/1476-511X-13-135
11. Koob T., et al. Cytokines in single layer amnion allografts compared to multilayer amnion/chorion allografts for wound healing. Journal of Biomedical Materials Research Part B: Applied Biomaterials. Aug 2014, epub ahead of print. DOI: 10.1002/jbm.b.33265
12. Kiliç Z., et al. Circulating matrix metalloproteinases and tissue inhibitors of metalloproteinases levels in pediatric patients with congenital heart disease: Relationship to cardiac functions. Anadolu Kardiyol Derg 2014, 14: 531-41. DOI:10.5152/akd.2014.4950
13. Rolin G., et al. In vitro study of the impact of mechanical tension on the dermal fibroblast henotype in the context of skin wound healing. Journal of Biomechanics Sept 2014, epub ahead of print. http://dx.doi.org/10.1016/j.jbiomech.2014.07.015

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 1 x 96-Well Strip Microplate Kit
Lieferbar: In stock
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