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Human Leptin ELISA

Human Leptin ELISA

ArtNr	ELH-Leptin-5
Hersteller	Raybiotech
Menge	5 Plate Kit
Quantity options	1 x 96-Well Strip Microplate Kit 2 Plate Kit 5 Plate Kit
Kategorie	Oncology Metabolism Diabetes Glucose Homeostasis Cholesterol & Lipid Metabolism Regulation of Appetite Obesity ELISA Endocrine Regulation of Lipid Metabolism ELISA & Immunoassays ELISA Kits Immunoassay By Organ Bladder Cancer Breast Cancer
Typ	Elisa-Kit
Specific against	Human
Host	Hamster - Armenian
Sensitivity	2 pg/ml
Citations	1. Wang B H, Trayhurn P. Acute and prolonged effects of TNF-alpha on the expression and secretion of inflammation-related adipokines by human adipocytes differentiated in culture. Pflugers Arch - Eur J Physiol. 2006,452:418–427<br />2. Dubois V, Delort L, Billard H, et al. Breast cancer and obesity: in vitro interferences between adipokines and proangiogenic features and/or antitumor therapies? PLoS ONE. 2013,8(3):e58541. doi:10.1371/journal.pone.0058541<br />3. Dubois V, Delort L, Billard H, et al. Breast cancer and obesity: in vitro interferences between adipokines and proangiogenic features and/or antitumor therapies? PLoS ONE. 2013,8(3):e58541.<br />4. Associations between perinatal factors and adiponectin and leptin in 9-year-old Mexican-American children<br />5. Dubois V, Delort L, Billard H, et al. Breast cancer and obesity: in vitro interferences between adipokines and proangiogenic features and/or antitumor therapies? PLoS ONE. 2013,8(3):e58541. doi:10.1371/journal.pone.0058541<br />6. Dubois V, Delort L, Billard H, et al. Breast cancer and obesity: in vitro interferences between adipokines and proangiogenic features and/or antitumor therapies? PLoS ONE. 2013,8(3):e58541.<br />7. Gileles-Hillel A., Alonso-Alvares M., Kheirandish-Gozal L., Peris E., et al. Inflammatory markers and obstructive sleep apnea in obese children: The NANOS Study. Mediators of Inflammation. Accepted May 2014 http://www.hindawi.com/journals/mi/aip/605280/ [Epub ahead of print]<br />8. Bil-Lula I., et al. Is There any Link Between Visceral Obesity and Adenovirus Infections in the Polish Population? Adv Clin Exp Med 2014, 23, 3, 415–422<br />9. Bhaktha G., et al. SERUM TNF-alpha AND LEPTIN LEVELS IN GRADED OBESE DIABETIC WOMEN- FREE FROM MICRO AND MACRO VASCULAR COMPLICATIONS. IJPCBS 2014, 4(3), 648-653<br />10. Ha AW., et al. Plasma adipocytokines and antioxidants-status in Korean overweight and obese females with dyslipidemia. Nutr Res Pract. 2014 Aug,8(4):417-424. http://dx.doi.org/10.4162/nrp.2014.8.4.417 <br />11. Lin Y., et al. Dietary fiber intake and its association with indicators of adiposity and serum biomarkers in European adolescents: the HELENA study. European Journal of Nutrition August 2014, Epub Ahead of Print. DOI 10.1007/s00394-014-0756-2<br />12. Bil-lula I., et al. Infectobesity in the Polish Population - Evaluation of an Association between Adenoviruses Type 5, 31, 36 and Human Obesity. International Journal of Virology and Molecular Biology 2014, 3(1): 1-8. DOI: 10.5923/j.ijvmb.20140301.01
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Similar products	Leptin
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Specificity	The Leptin ELISA kit shows no cross-reactivity with any of the cytokines tested: Human Angiogenin, BDNF, BLC, ENA-78, FGF-4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, G
Quantity options
Compatible Sample Types	Cell Culture Supernatants, Plasma, Serum, Urine
Design Principle	Sandwich-based
Kit Components	Pre-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate
Method of Detection	Colorimetric
Other Materials Required	Distilled or deionized water Precision pipettes to deliver 2 ul to 1 ul volumes Adjustable 1-25 ul pipettes for reagent preparation 100 ul and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis
Product Features	Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
Protocol Summary	Prepare all reagents, samples and standards as instructed in the manual. Add 100 ul of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4C. Add 100 ul of prepared biotin antibody to each well. Incubate 1 h at RT. Add 100 ul of prepared Streptavidin solution to each well. Incubate 45 min at RT. Add 100 ul of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 ul of Stop Solution to each well. Read at 450 nm immediately.
Recommended Dilution (serum & plasma)	20-50 fold
Result Output	Quantitative
Short description	RayBio Human Leptin (OB) ELISA Kit for serum, plasma, cell culture supernatants, and urine
Solid Support	96-well Microplate
Storage	May be stored for up to 6 months at 2C to 8C from the date of shipment. Standard (recombinant protein) should be stored at -20C or -80C (recommended at -80C) after reconstitution. Opened Microplate Wells or reagents may be store for up to 1 month at 2C to 8C. Return unused wells to the pouch containing desiccant pack, reseal along entire edge. Note: the kit can be used within one year if the whole kit is stored at -20C.
Target Name and Synonyms	Leptin

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