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Human Clusterin ELISA Kit

Human Clusterin ELISA Kit

ArtNr	ELH-Clusterin-1
Hersteller	Raybiotech
Menge	1 Plate Kit
Quantity options	1 Plate Kit 2 Plate Kit 5 Plate Kit
Kategorie	Oncology Neuroscience Metabolism Cholesterol & Lipid Metabolism Molecular Targets for Neuroscience Protein Aggregation, Clearance & Misfolding By Cell Type Carcinoma ELISA Lipid & Fatty Acid Transport ELISA & Immunoassays ELISA Kits Immunoassay Other Lipid\Cholesterol Metabolism Modulators
Typ	Elisa-Kit
Specific against	Human (Homo sapiens)
Host	Hamster - Armenian
Sensitivity	15 pg/ml
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Similar products	Clusterin
Versandbedingung	Gekühlt
Lieferbar
Specificity	This ELISA antibody pair detect human Clusterin. Other species not determined.
Manufacturer - Category	ELISA Kits\|Sandwich ELISA
Manufacturer - Targets	Clusterin
Shipping Temperature	Blue ice
Storage Conditions	?-20°C
Description	RayBio® Human Clusterin ELISA Kit for cell culture supernatants, plasma, and serum samples. Lead time: Typically ships within 1-2 business days. No Friday shipments.
Compatible Sample Types	Cell Culture Supernatants, Plasma, Serum, Urine
Design Principle	Sandwich-based
Method of Detection	Colorimetric
Solid Support	96-well Microplate
Kit Components	Pre-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate
Other Materials Required	Distilled or deionized water Precision pipettes to deliver 2 ul to 1 ul volumes Adjustable 1-25 ul pipettes for reagent preparation 100 ul and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline	Prepare all reagents, samples and standards as instructed in the manual. Add 100 ul of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4C. Add 100 ul of prepared biotin antibody to each well. Incubate 1 h at RT. Add 100 ul of prepared Streptavidin solution to each well. Incubate 45 min at RT. Add 100 ul of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 ul of Stop Solution to each well. Read at 450 nm immediately.
Product Features	Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
Result Output	Quantitative
UNSPSC Code	41116158
Manufacturer - Specificity	This ELISA antibody pair detect human Clusterin. Other species not determined.
Recommended Dilution	100, 000 fold

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