Human Autoimmune Disease IgG Autoantibody Array G1

33

Sandwich-based

Semi-Quantitative

CALCA | CENPA | CENPB | GARS | HARS | KRT19 | KRT20 | KRT8 | MPO | NUP210|Â NUP62 | PCNA | PRTN3 | SNRNP70 | SP100 | SSB | TARS2 | TG | TGM2 | THPO | TOP1 | TRIM21

Small plastic boxes or containers
Orbital shaker or oscillating rocker
Pipettors, pipette tips and other common lab consumables
Distilled water
Aluminum foil
Laser scanner for fluorescence detection

Small sample volume required (2 µl)
High density (simultaneously detect 33 autoantibodies)
High sensitivity
Large dynamic range
Suitable for high-throughput assays
High efficiency and accuracy
Affordable, quick and simple to use
Customizable: Tell us the autoimmunogens you're interested in

The following Figure 1 represents a typical result using serum on the RayBio® Autoimmune Disease IgG Autoantibody Detection Array (Cat #. PAH-AIDG-G1). The protein arrays were incubated with 200-fold diluted serum samples from normal individuals and patients with different autoimmune diseases. After washing steps, the arrays were sequentially incubated with biotin-labeled anti-human IgG antibody and fluorescent dye-labeled streptavidin. The signals from the bound autoantibodies were captured with a fluorescence scanner.
Sample diluent alone (Figs. 1A & 1B) and normal sera (Figs. 1C & 1D) were used as negative controls, and the arrays signals indicate that no significant background occurred. In some cases, a negligible signal can be observed, which may be caused by non-specific binding derived from the biotin-labeled anti-human IgG antibody and/or fluorescent dye-labeled streptavidin. However, these weak background signals will be subtracted in subsequent data analysis procedures and will not affect sample test results. A strong binding signal was detected in multiple targets among different autoimmune disease patients: systemic lupus erythematosus (Figs.1E & 1F), Sjøgren's syndrome (Figs.1G & 1H), system vasculitis (Figs.1I & 1J), and primary biliary cirrhosis (Figs.1K & 1L).
Fig 1. Typical result using serum on the RayBio® Autoimmune Disease IgG Autoantibody Detection Array. The protein arrays were incubated with diluent alone (A, B), diluted serum samples derived from normal individuals (C, D) and those from various autoimmune disease patients (E-L). Yellow boxes, show various controls as indicated (biotin-labeled BSA, human IgG and IgA).