Vergleich

Mouse / Rat Urotensin-2 ELISA Kit

ArtNr EIAM-UTS2-2
Hersteller Raybiotech
Menge 2 Plate Kit
Quantity options 1 Plate Kit 2 Plate Kit 5 Plate Kit
Kategorie
Typ EIA
Specific against Mouse (Murine, Mus musculus), Rat (Rattus norvegicus)
Host Hamster - Armenian
Sensitivity 0.3 ng/ml
ECLASS 10.1 32161000
ECLASS 11.0 32161000
UNSPSC 41116126
Versandbedingung Gekühlt
Lieferbar
Specificity This EIA kit is designed to detect mouse, and rat Urotensin-2
Compatible Sample Types
Cell Culture Supernatants
Plasma
Serum
Design Principle
Competition-based
Method of Detection
Colorimetric
Quantitative/Semi-Quantitative
Quantitative
Solid Support
96-well Microplate
Gene Symbols
UCN2UTS2
Kit Components
Pre-
Coated 96-well Strip Microplate
Wash Buffer
Standard Peptide
Assay Diluent(s)
Biotinylated Peptide
H
R
P-
Streptavidin
T
M
B One-
Step Substrate
Stop Solution
Assay Diagram
Positive Control Sample
Capture Antibody
User Manual
Other Materials Required
Distilled or deionized water
Precision pipettes to deliver 2 µl to 1 ml volumes
Adjustable 1-25 ml pipettes for reagent preparation100 ml and 1 liter graduated cylinders
Tubes to prepare standard and sample dilutions
Orbital shaker
Aluminum foil
Saran Wrap
Absorbent paper
Microplate reader capable of measuring absorbance at 450nm
Sigma
Plot software (or other software that can perform four-parameter logistic regression models)
Protocol Outline
Prepare all reagents, samples and standards as instructed.
Add 100 µl detection antibody to each well.
Incubate 1.5 h at R
T or O/
N at 4°
C.
Add 100 µl standard or sample to each well.
Incubate 2.5 h at R
T.
Add 100 µl prepared streptavidin solution.
Incubate 45 min at R
T.
Add 100 µl T
M
B One-
Step Substrate Reagent to each well.
Incubate 30 min at R
T.
Add 50 µl Stop Solution to each well.
Read plate at 450 nm immediately.
Product Features
Strip plates and additional reagents allow for use in multiple experiments
Quantitative protein detection
Establishes normal range
The best products for confirmation of antibody array data
Protein Name & Synonyms
Urotensin-2 (Urotensin II) (U-II) (UII)
Specicifity
This EIA kit is designed to detect mouse, and rat Urotensin-2
Recommended Dilution (serum & plasma)
Mouse: 2X / Rat: 2X

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 2 Plate Kit
Lieferbar: In stock
lieferbar

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