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Mouse Apoptosis Signaling Pathway Array C1

Mouse Apoptosis Signaling Pathway Array C1

ArtNr	AAM-APOSIG-1-2
Hersteller	Raybiotech
Menge	2 Sample Kit
Quantity options	2 Sample Kit 4 Sample Kit 8 Sample Kit
Kategorie	Cardiovascular Research Ischemia & Reperfusion Injury Arrays Antibody Arrays
Typ	Array
Specific against	Mouse (Murine, Mus musculus)
Host	Hamster - Armenian
ECLASS 10.1	32161000
ECLASS 11.0	32161000
UNSPSC	41116126
Versandbedingung	Gekühlt
Lieferbar
Manufacturer - Applications	Multiplexed Protein Detection Detection of Relative Protein Expression Detecting Patterns of Cytokine Expression Biomarker/ Key Factor Screening Identifying Key Factors Confirming a Biological Process
Number of Targets Detected	17
Compatible Sample Types	Cell Culture Supernatants Cell Lysates Plasma Serum Tissue Lysates
Design Principle	Sandwich-based
Method of Detection	Chemiluminescence
Quantitative/Semi-Quantitative	Semi-Quantitative
Solid Support	Membrane
Gene Symbols	AKT1 \| ATM \| BAD \| CASP3 \| CASP7 \| CDKN1B \| CHEK1 \| EIF2S1 \| HSPB1 \| MAP3K7\|Â MAPK14 \| MAPK8 \| NFKBIA \| RELA \| SMAD2 \| TP53
Kit Components	Mouse Apoptosis Signaling Pathway Antibody Array C1 Membranes Blocking Buffer Detection Antibody Cocktail1, 000 X H R P- Anti- Rabbit Ig G Concentrate20 X Wash Buffer I Concentrate20 X Wash Buffer I I Concentrate2 X Cell Lysis Buffer Concentrate Detection Buffer C Detection Buffer D8- Well Incubation Tray w/ Lid Protease Inhibitor Cocktail100x Phosphatase Inhibitor Cocktail I Phosphatase Inhibitor Cocktail I I Plastic Sheets Array Map Template Manual
Other Materials Required	Pipettors, pipet tips and other common lab consumables Orbital shaker or oscillating rocker Tissue Paper, blotting paper or chromatography paper Adhesive tape or Saran Wrap Distilled or de-ionized water A chemiluminescent blot documentation system (such as U V P's Chemi Doc- ItÂ® or Epi Chem I I Benchtop Darkroom or G E's Image QuantÂ„Â¢ L A S 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline	Block membranes Incubate with Sample Incubate with Detection Antibody Cocktail Incubate with H R P- Conjugated anti- Ig G Incubate with Detection Buffers Image with chemiluminescent imaging system Perform densitometry and analysis
Storage/Stability	For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
Pathway	Akt SignalingHER/ErbB SignalingHIF-1 alpha SignalingIGF SignalingJAK/STAT SignalingMAPK SignalingmTOR SignalingNFkB Signalingp53 SignalingPI3K-AKT SignalingPKC SignalingTGF-beta Signaling

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