ArtNr |
AAM-APO-1-2 |
Hersteller |
Raybiotech
|
Menge |
2 Sample Kit |
Quantity options |
2 Sample Kit
4 Sample Kit
8 Sample Kit
|
Kategorie |
|
Typ |
Array |
Specific against |
Mouse |
Host |
Hamster - Armenian |
ECLASS 10.1 |
32161000 |
ECLASS 11.0 |
32161000 |
UNSPSC |
41116126 |
Versandbedingung |
Gekühlt |
Lieferbar |
|
Number of Targets Detected |
38 |
Species Detected |
Mouse |
Compatible Sample Types |
Cell Culture Supernatants Cell Lysates Plasma Serum Tissue Lysates |
Design Principle |
Sandwich-based |
Method of Detection |
Chemiluminescence |
Quantitative/Semi-Quantitative |
Semi-Quantitative |
Solid Support |
Membrane |
Gene Symbols |
BAD | BAX | BCL2 | BCL2L11 | BCL2L2 | BID | BIRC3 | BIRC5 | CASP3 | CASP8| CD40 | CD40LG | CDKN1A | DIABLO | FAS | FASLG | HSPA4 | HSPB1 | HSPD1 | HTRA2 | IGF1 | IGF2 | IGFBP1 | IGFBP2 | IGFBP3 | IGFBP4 | IGFBP5 | IGFBP6 | LTA | PER27 | TNF | TNFRSF10B | TNFRSF1A | TNFRSF1B | TNFRSF21 | TP53 | XIAP |
Application Information |
Multiplexed Protein Detection Detection of Relative Protein Expression Detecting Patterns of Cytokine Expression Biomarker/ Key Factor Screening Identifying Key Factors Confirming a Biological Process |
Kit Components |
Mouse Apoptosis Antibody Array C1 Membranes Blocking Buffer Wash Buffer 1 Wash Buffer 2 Biotinylated Detection Antibody Cocktail Streptavidin- Conjugated H R P Detection Buffer C Detection Buffer D Lysis Buffer Protease Inhibitor Cocktail8- Well Incubation Tray Plastic Sheets Array Templates Manual |
Other Materials Required |
Pipettors, pipet tips and other common lab consumables Orbital shaker or oscillating rocker Tissue Paper, blotting paper or chromatography paper Adhesive tape or Saran Wrap Distilled or de-ionized water A chemiluminescent blot documentation system (such as U V P's Chemi Doc- It® or Epi Chem I I Benchtop Darkroom or G E's Image Quant™ L A S 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system. |
Protocol Outline |
Block membranes Incubate with Sample Incubate with Biotinylated Detection Antibody Cocktail Incubate with H R P- Conjugated Streptavidin Incubate with Detection Buffers Image with chemiluminescent imaging system Perform densitometry and analysis |
Product Features |
Easy to use No specialized equipment needed Compatible with nearly any liquid sample Proven technology (many publications) Highly sensitive (pg/ml) Sandwich ELISA specificity Higher density than ELISA, Western blot or bead-based multiplex |
Pathway |
Akt SignalingIGF SignalingMAPK SignalingNFkB Signalingp53 SignalingPI3K-AKT Signaling |
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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.