ArtNr |
QAR-CYT-2-2 |
Hersteller |
Raybiotech
|
Menge |
22 Sample Kit |
Quantity options |
8 Sample Kit
22 Sample Kit
50 Sample Kit
|
Kategorie |
|
Typ |
Array |
Specific against |
Rat (Rattus norvegicus) |
Host |
Hamster - Armenian |
Citations |
Driesen R., et al. Reversible and irreversible differentiation of cardiac fibroblasts. Cardiovascular Research (2014) 101, 411-422. DOI:10.1093/cvr/cvt338 |
ECLASS 10.1 |
32161000 |
ECLASS 11.0 |
32161000 |
UNSPSC |
41116126 |
Similar products |
IFN-gamma, IL-10, IL-1 beta, IL-6, Leptin, TIMP-1, MCP-1, ICAM-1, TNF alpha, L-selectin |
Versandbedingung |
Gekühlt |
Lieferbar |
|
Number of Targets Detected |
10 |
Compatible Sample Types |
Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates |
Design Principle |
Sandwich-based |
Solid Support |
Glass Slide |
Kit Components |
- Rat Cytokine Array Q2 Slide(s)
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Lyophilized Standard Mix
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated Fluor
- 30 ml Centrifuge Tube
- Adhesive Plastic Strips
- Manual
|
Other Materials Required |
- Distilled or deionized water
- Small plastic boxes or containers
- Pipettors, pipette tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Aluminum foil
- Gene microarray scanner or similar laser fluorescence scanner
|
Protocol Outline |
- Dry the glass slide
- Prepare Standards
- Block array surface
- Incubate with Samples and Standards
- Incubate with Biotinylated Detection Antibody Cocktail
- Incubate with Streptavidin-Conjugated Fluor
- Disassemble the glass slide
- Scan with a gene microarray laser scanner
- Perform densitometry and analysis
|
Detection Method |
Fluorescence Laser Scanner |
Features |
- More cost-effective than traditional ELISA
- High specificity and system reproducibility
- Suitable for diverse sample types
- Low sample volume requirement: 50 uL or less
- Get results same day (6-hour processing time)
- Well-suited for high throughput assays
- Q Analyzer software provides one-step computation
|
Result Output |
Quantitative |
Short description |
Quantibody Rat Cytokine Array 2 Kit. Detects 10 Rat Cytokines. Suitable for all liquid sample types. |
Storage |
For best results, store the entire kit frozen at -20C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s), standard mix, detection antibody cocktail, and Cy3-Conjugated Streptavidin at -20C and all other reagents undiluted at 4C for no more than 3 months. |
Targets Detected |
CCL2, CD54, ICAM-1, IFN-gamma, IL-1 beta, IL-1 F2, IL-10, IL-6, L-Selectin, Leptin, MCP-1, TIMP-1, TNF alpha |
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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.