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Rat Cytokine Array Q2

Rat Cytokine Array Q2

ArtNr	QAR-CYT-2-1
Hersteller	Raybiotech
Menge	8 Sample Kit
Quantity options	8 Sample Kit 22 Sample Kit 50 Sample Kit
Kategorie	Oncology Neuroscience Neural Lineage Markers Neuroinflammation Metabolism Diabetes Glucose Homeostasis Cholesterol & Lipid Metabolism Regulation of Appetite Neural Signaling By Cell Type Carcinoma Hematology Leukemia Myeloma Cell Biology Cell Movement Cell Adhesion Obesity Cell Culture Cytokines Cell Status Necrosis Endocrine Regulation of Lipid Metabolism Arrays Antibody Arrays
Typ	Array
Specific against	Rat
Host	Hamster - Armenian
Citations	Driesen R., et al. Reversible and irreversible differentiation of cardiac fibroblasts. Cardiovascular Research (2014) 101, 411-422. DOI:10.1093/cvr/cvt338
ECLASS 10.1	32161000
ECLASS 11.0	32161000
UNSPSC	41116126
Similar products	IFN-gamma, IL-10, IL-1 beta, IL-6, Leptin, TIMP-1, IL-6, MCP-1, ICAM-1, TNF alpha, L-selectin
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Short description	Quantibody Rat Cytokine Array 2 Kit. Detects 10 Rat Cytokines. Suitable for all liquid sample types.
Features	More cost-effective than traditional ELISA High specificity and system reproducibility Suitable for diverse sample types Low sample volume requirement: 50 uL or less Get results same day (6-hour processing time) Well-suited for high throughput assays Q Analyzer software provides one-step computation
Number of Targets Detected	10
Target Names	ICAM-1 (CD54), IFN-gamma, IL-1 beta (IL-1 F2), IL-10, IL-6, Leptin, L-Selectin (CD62L), MCP-1 (CCL2), TIMP-1, TNF alpha
Targets Detected	CCL2, CD54, ICAM-1, IFN-gamma, IL-1 beta, IL-1 F2, IL-10, IL-6, L-Selectin, Leptin, MCP-1, TIMP-1, TNF alpha
Suggested Applications	Multiplexed Protein Detection, Biomarker Screening, Identifying Key Factors, Confirming Biological Process, Biomarker Validation, Validation of Antibody Array Results, Quantitative Protein Detection, Establishing Normal Range
Kit Components	Rat Cytokine Array Q2 Slide(s) Blocking Buffer Wash Buffer 1 Wash Buffer 2 Lyophilized Standard Mix Biotinylated Detection Antibody Cocktail Streptavidin-Conjugated Fluor 30 ml Centrifuge Tube Adhesive Plastic Strips Manual
Other Materials Required	Distilled or deionized water Small plastic boxes or containers Pipettors, pipette tips and other common lab consumables Orbital shaker or oscillating rocker Aluminum foil Gene microarray scanner or similar laser fluorescence scanner
Protocol Outline	Dry the glass slide Prepare Standards Block array surface Incubate with Samples and Standards Incubate with Biotinylated Detection Antibody Cocktail Incubate with Streptavidin-Conjugated Fluor Disassemble the glass slide Scan with a gene microarray laser scanner Perform densitometry and analysis
Storage	For best results, store the entire kit frozen at -20C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s), standard mix, detection antibody cocktail, and Cy3-Conjugated Streptavidin at -20C and all other reagents undiluted at 4C for no more than 3 months.
Solid Support	Glass Slide
Design Principle	Sandwich-based
Result Output	Quantitative
Detection Method	Fluorescence Laser Scanner
Compatible Sample Types	Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates
Citation	Driesen R., et al. Reversible and irreversible differentiation of cardiac fibroblasts. Cardiovascular Research (2014) 101, 411-422. DOI:10.1093/cvr/cvt338

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