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Rat Cytokine Array C2

ArtNr AAR-CYT-2-4
Hersteller Raybiotech
Menge 4 Sample Kit
Quantity options 2 Sample Kit 4 Sample Kit 8 Sample Kit
Kategorie
Typ Array
Specific against Rat
Host Hamster - Armenian
Citations Wu, F., Zhang, R. et al. Preliminary Study on Pain Reduction of Monosodium Iodoacetate-Induced Knee Osteoarthritis in Rats by Carbon Dioxide Laser Moxibustion. Evidence-Based Complementary and Alternative Medicine 2014. Epub Ahead of Print. DOI: http://dx.doi.org/10.1155/2014/754304
ECLASS 10.1 32161000
ECLASS 11.0 32161000
UNSPSC 41116126
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Lieferbar
Compatible Sample Types
Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates
Design Principle
Sandwich-based
Detection Method
Chemiluminescence
Features
  • Easy to use
  • No specialized equipment needed
  • Compatible with nearly any liquid sample
  • Proven technology (many publications)
  • Highly sensitive (pg/ml)
  • Sandwich ELISA specificity
  • Higher density than ELISA, Western blot or bead-based multiplex
Kit Components
  • Rat Cytokine Antibody Array C2 Membranes
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated HRP
  • Detection Buffer C
  • Detection Buffer D
  • Lysis Buffer
  • 8-Well Incubation Tray
  • Plastic Sheets
  • Array Templates
  • Manual
Number of Targets Detected
34
Other Materials Required
  • Pipettors, pipet tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Tissue Paper, blotting paper or chromatography paper
  • Adhesive tape or Saran Wrap
  • Distilled or de-ionized water
  • A chemiluminescent blot documentation system (such as UVPs ChemiDoc-It or EpiChem II Benchtop Darkroom), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline
  1. Block membranes
  2. Incubate with Sample
  3. Incubate with Biotinylated Detection Antibody Cocktail
  4. Incubate with HRP-Conjugated Streptavidin
  5. Incubate with Detection Buffers
  6. Image with chemiluminescent imaging system
  7. Perform densitometry and analysis
Result Output
Semi-Quantitative
Short description
RayBio C-Series Rat Cytokine Antibody Array 2 Kit. Detects 34 Rat Cytokines. Suitable for all liquid sample types.
Solid Support
Membrane
Storage
For best results, store the entire kit frozen a -20C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer a -20C and all other reagents undiluted at 4C for no more than 3 months.
Targets Detected
Activin A, Agrin, B7-2, beta-NGF, CCL2, CCL20, CD54, CD62L, CD86, CINC-1, CINC-2, CINC-3, CNTF, CX3CL1, CXCL1, CXCL7, Fas Ligand, Fractalkine, GM-CSF, ICAM-1, IFN-gamma, IL-1 alpha, IL-1 beta, IL-1 F1, IL-1 F2, IL-1 R6, IL-1 Rrp2, IL-10, IL-13, IL-2, IL-4, IL-6, L-Selectin, Leptin, LIX, MCP-1, MIP-3 alpha, MMP-8, PDGF-AA, Prolactin R, RAGE, TCK-1, TIMP-1, TNF alpha, TNFSF6, VEGF-A

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 4 Sample Kit
Lieferbar: In stock
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