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Rat Cytokine Array C2

Rat Cytokine Array C2

ArtNr	AAR-CYT-2-4
Hersteller	Raybiotech
Menge	4 Sample Kit
Quantity options	2 Sample Kit 4 Sample Kit 8 Sample Kit
Kategorie	Oncology Neuroscience Neural Lineage Markers Neuroinflammation Metabolism Diabetes Glucose Homeostasis Cholesterol & Lipid Metabolism Regulation of Appetite Molecular Targets for Neuroscience Targets for Rodent Neurosciences Neural Signaling By Cell Type Carcinoma Glioma Hematology Leukemia Lymphoma Myeloma Cell Biology Cell Movement Cell Adhesion Obesity Cell Culture Cytokines Cell Status Necrosis Apoptosis Adenocarcinoma Endocrine Regulation of Lipid Metabolism Enzymes Fatty Acid Synthesis Arrays By Organ Bladder Cancer Antibody Arrays
Typ	Array
Specific against	Rat (Rattus norvegicus)
Host	Hamster - Armenian
Citations	Wu, F., Zhang, R. et al. Preliminary Study on Pain Reduction of Monosodium Iodoacetate-Induced Knee Osteoarthritis in Rats by Carbon Dioxide Laser Moxibustion. Evidence-Based Complementary and Alternative Medicine 2014. Epub Ahead of Print. DOI: http://dx.doi.org/10.1155/2014/754304
ECLASS 10.1	32161000
ECLASS 11.0	32161000
UNSPSC	41116126
Similar products	IFN-gamma, IL-10, IL-1 alpha, IL-1 beta, IL-6, Leptin, TIMP-1, IL-13, IL-4, Fractalkine, Agrin, CNTF, VEGF-A, IL-2, RAGE, GM-CSF, MCP-1, Activin A, ICAM-1, LIX, PDGF-AA, MIP-3 alpha, TNF alpha, beta-NGF, MMP-8, Fas Ligand, L-Selectin, Prolactin R, IL-1 R6, CINC-3, CINC-1, B7-2 (CD86), CINC-2 alpha, Thymus Chemokine-1 (CXCL7)
Versandbedingung	Gekühlt
Lieferbar
Number of Targets Detected	34
Compatible Sample Types	Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates
Design Principle	Sandwich-based
Solid Support	Membrane
Kit Components	Rat Cytokine Antibody Array C2 Membranes Blocking Buffer Wash Buffer 1 Wash Buffer 2 Biotinylated Detection Antibody Cocktail Streptavidin-Conjugated HRP Detection Buffer C Detection Buffer D Lysis Buffer 8-Well Incubation Tray Plastic Sheets Array Templates Manual
Other Materials Required	Pipettors, pipet tips and other common lab consumables Orbital shaker or oscillating rocker Tissue Paper, blotting paper or chromatography paper Adhesive tape or Saran Wrap Distilled or de-ionized water A chemiluminescent blot documentation system (such as UVPs ChemiDoc-It or EpiChem II Benchtop Darkroom), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline	Block membranes Incubate with Sample Incubate with Biotinylated Detection Antibody Cocktail Incubate with HRP-Conjugated Streptavidin Incubate with Detection Buffers Image with chemiluminescent imaging system Perform densitometry and analysis
Detection Method	Chemiluminescence
Features	Easy to use No specialized equipment needed Compatible with nearly any liquid sample Proven technology (many publications) Highly sensitive (pg/ml) Sandwich ELISA specificity Higher density than ELISA, Western blot or bead-based multiplex
Result Output	Semi-Quantitative
Short description	RayBio C-Series Rat Cytokine Antibody Array 2 Kit. Detects 34 Rat Cytokines. Suitable for all liquid sample types.
Storage	For best results, store the entire kit frozen a -20C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer a -20C and all other reagents undiluted at 4C for no more than 3 months.
Targets Detected	Activin A, Agrin, B7-2, beta-NGF, CCL2, CCL20, CD54, CD62L, CD86, CINC-1, CINC-2, CINC-3, CNTF, CX3CL1, CXCL1, CXCL7, Fas Ligand, Fractalkine, GM-CSF, ICAM-1, IFN-gamma, IL-1 alpha, IL-1 beta, IL-1 F1, IL-1 F2, IL-1 R6, IL-1 Rrp2, IL-10, IL-13, IL-2, IL-4, IL-6, L-Selectin, Leptin, LIX, MCP-1, MIP-3 alpha, MMP-8, PDGF-AA, Prolactin R, RAGE, TCK-1, TIMP-1, TNF alpha, TNFSF6, VEGF-A

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