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Glycan Array 100

Glycan Array 100

ArtNr	GA-Glycan-100-1
Hersteller	Raybiotech
Menge	4 Sample Kit
Quantity options	4 Sample Kit 8 Sample Kit 16 Sample Kit
Kategorie	Arrays Protein Arrays
Typ	Array
Specific against	other
Host	Hamster - Armenian
Citations	Amonsen, M., Smith, D. F., Cummings, R. D., Air, G. M. J Virol 2007, 81, 8341-8345 Paulson, J. C., de Vries, R. P. Virus Res 2013, 178, 99-113 Stencel-Baerenwald, J. E., Reiss, K., Reiter, D. M., Stehle, T., Dermody, T. S. Nat Rev Microbiol 2014, 12, 739-749 Park, S., Shin, I. Org Lett 2007, 9, 1675-1678 Pourceau, G., Chevolot, Y., Goudot, A., Giroux, F., Meyer, A., Moules, V., Lina, B., Cecioni, S., Vidal, S., Yu, H., Chen, X., Ferraris, O., Praly, J. P., Souteyrand, E., Vasseur, J. J., Morvan, F. Chembiochem 2011, 12, 2071-2080 Laurent, N., Voglmeir, J., Flitsch, S. L. Chem Commun 2008, 4400-4412 Xu, R., de Vries, R. P., Zhu, X., Nycholat, C. M., McBride, R., Yu, W., Paulson, J. C., Wilson, I. A. Science 2013, 342, 1230-1235 Xu, R., McBride, R., Nycholat, C. M., Paulson, J. C., Wilson, I. A. J Virol 2012, 86, 982-990 Stevens, J., Chen, L. M., Carney, P. J., Garten, R., Foust, A., Le, J., Pokorny, B. A., Manojkumar, R., Silverman, J., Devis, R., Rhea, K., Xu, X., Bucher, D. J., Paulson, J. C., Cox, N. J., Klimov, A., Donis, R. O. J Virol 2010, 84, 8287-8299 Stowell, S. R., Arthur, C. M., Mehta, P., Slanina, K. A., Blixt, O., Leffler, H., Smith, D. F., Cummings, R. D. J Biol Chem 2008, 283, 10109-10123 Stowell, S. R., Arthur, C. M., Slanina, K. A., Horton, J. R., Smith, D. F., Cummings, R. D. J Biol Chem 2008, 283, 20547-20559 Carlsson, S., Oberg, C. T., Carlsson, M. C., Sundin, A., Nilsson, U. J., Smith, D., Cummings, R. D., Almkvist, J., Karlsson, A., Leffler, H. Glycobiology 2007, 17, 663-676.
ECLASS 10.1	32161000
ECLASS 11.0	32161000
UNSPSC	41116126
Lieferbar
Number of Targets Detected	100
Compatible Sample Types	Cell Culture Supernatants Cell Lysates Plasma Purified Proteins Serum Tissue Lysates
Design Principle	Label-basedSandwich-based
Method of Detection	Fluorescence Laser Scanner
Quantitative/Semi-Quantitative	Semi-Quantitative
Solid Support	Glass Slide
Kit Components	Dialysis Vials Labeling Reagent Stop Solution Glycan Array Glass Slide Assembly Sample Diluent20 X Wash Buffer I20 X Wash Buffer I I Cy3 equivalent dye-conjugated Streptavidin Slide Washer/ Dryer Adhesive device sealer Labeling Buffer Floating Dialysis Rack
Other Materials Required	Detection antibodies of interest ( For sandwich-based method only) Pipettors, pipet tips and other common lab consumables Orbital shaker or oscillating rocker Aluminum foil Distilled or de-ionized water1.5ml Polypropylene microcentrifuge tubes Gene microarray scanner or similar laser fluorescence scanner Small plastic or glass containers K Cl, Na Cl, K H2 P O4 and Na2 H P O4 ( For label-based method only) Beaker, stir plate and stir bar
Protocol Outline	Dry the glass slide Block array surfaces Incubate samples (samples need to be biotinylated for the label-based approach) For the the sandwich-based principle, incubate with a detection antibody cocktail For the label-based principle, incubate the labeled-streptavidin. Incubate with Cy3 Equivalent Dye- Streptavidin Disassemble the glass slide Scan with a gene microarray laser scanner Perform densitometry and analysis
Introduction	Glycocalyx, literally meaning ‘sugar coat’, is an extracellular polymeric coating surrounding many prokaryotic and eukaryotic cells consisting of glycoproteins, glycolipids, proteoglycans and glycosaminoglycans. The constituents of the glycocalyx play an important role in the process of cell signaling, virus infections, and immunity. However, detection tools for the research of glycobiology are currently in very limited supply.Raybiotech has pioneered the development of antibody arrays which are now widely applied in the research community with thousands of peer reviewed publications, including Cell and Nature. Taking advantage of advancements in microarray technology developed for antibody arrays, we have developed the first commercially available glycan array for screening protein-carbohydrate interactions. This array will help researchers
References	Amonsen, M. Smith, D. F. Cummings, R. D. Air, G. M. J Virol 2007, 81, 8341-8345. Paulson, J. C. de Vries, R. P. Virus Res 2013, 178, 99-113. Stencel-Baerenwald, J. E. Reiss, K. Reiter, D. M. Stehle, T. Dermody, T. S. Nat Rev Microbiol 2014, 12, 739-749. Park, S. Shin, I. Org Lett 2007, 9, 1675-1678. Pourceau, G. Chevolot, Y. Goudot, A. Giroux, F. Meyer, A. Moules, V. Lina, B. Cecioni, S. Vidal, S. Yu, H. Chen, X. Ferraris, O. Praly, J. P. Souteyrand, E. Vasseur, J. J. Morvan, F. Chembiochem 2011, 12, 2071-2080. Laurent, N. Voglmeir, J. Flitsch, S. L. Chem Commun 2008, 4400-4412. Xu, R. de Vries, R. P. Zhu, X. Nycholat, C. M. McBride, R. Yu, W. Paulson, J. C. Wilson, I. A. Science 2013, 342, 1230-1235. Xu, R. McBride, R. Nycholat, C. M. Paulson, J. C. Wilson, I. A. J Virol 2012, 86, 982-990. Stevens, J. Chen, L. M. Carney, P. J. Garten, R. Foust, A. Le, J. Pokorny, B. A. Manojkumar, R. Silverman, J. Devis, R. Rhea, K. Xu, X. Bucher, D. J. Paulson, J. C. Cox, N. J. Klimov, A. Donis, R. O. J Virol 2010, 84, 8287-8299. Stowell, S. R. Arthur, C. M. Mehta, P. Slanina, K. A. Blixt, O. Leffler, H. Smith, D. F. Cummings, R. D. J Biol Chem 2008, 283, 10109-10123. Stowell, S. R. Arthur, C. M. Slanina, K. A. Horton, J. R. Smith, D. F. Cummings, R. D. J Biol Chem 2008, 283, 20547-20559. Carlsson, S. Oberg, C. T. Carlsson, M. C. Sundin, A. Nilsson, U. J. Smith, D. Cummings, R. D. Almkvist, J. Karlsson, A. Leffler, H. Glycobiology 2007, 17, 663-676.

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