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Human Growth Factor Array C1

Human Growth Factor Array C1

ArtNr	AAH-GF-1-2
Hersteller	Raybiotech
Menge	2 Sample Kit
Quantity options	2 Sample Kit 4 Sample Kit 8 Sample Kit
Kategorie	Arrays Antibody Arrays
Typ	Array
Specific against	Human (Homo sapiens)
Host	Hamster - Armenian
ECLASS 10.1	32161000
ECLASS 11.0	32161000
UNSPSC	41116126
Versandbedingung	Gekühlt
Lieferbar
Manufacturer - Applications	Multiplexed Protein Detection Detection of Relative Protein Expression Detecting Patterns of Cytokine Expression Biomarker/ Key Factor Screening Identifying Key Factors Confirming a Biological Process
Number of Targets Detected	41
Compatible Sample Types	Cell Culture Supernatants Cell Lysates Plasma Serum Tissue Lysates
Design Principle	Sandwich-based
Method of Detection	Chemiluminescence
Quantitative/Semi-Quantitative	Semi-Quantitative
Solid Support	Membrane
Gene Symbols	AREG \| CSF1 \| CSF1R \| CSF2 \| CSF3 \| EGF \| EGFR \| FGF2 \| FGF4 \| FGF6\|Â FGF7 \| FIGF \| FLT4 \| GDNF \| HBEGF \| HGF \| IGF1 \| IGF1R \| IGF2 \| IGFBP1 \| IGFBP2 \| IGFBP3 \| IGFBP4 \| IGFBP6 \| KDR \| KIT \| KITLG \| NGF \| NTF3 \| NTF4 \| PDGFA \| PDGFB \| PDGFRA \| PDGFRB \| PGF \| TGFA \| TGFB1 \| TGFB2 \| TGFB3 \| VEGFA
Kit Components	Human Growth Factor Antibody Array C1 Membranes Blocking Buffer Wash Buffer 1 Wash Buffer 2 Biotinylated Detection Antibody Cocktail Streptavidin- Conjugated H R P Detection Buffer C Detection Buffer D Lysis Buffer8- Well Incubation Tray Plastic Sheets Array Templates Manual
Other Materials Required	Pipettors, pipet tips and other common lab consumables Orbital shaker or oscillating rocker Tissue Paper, blotting paper or chromatography paper Adhesive tape or Saran Wrap Distilled or de-ionized water A chemiluminescent blot documentation system (such as U V P's Chemi Doc- ItÂ® or Epi Chem I I Benchtop Darkroom or G E's Image Quantâ„¢ L A S 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline	Block membranes Incubate with Sample Incubate with Biotinylated Detection Antibody Cocktail Incubate with H R P- Conjugated Streptavidin Incubate with Detection Buffers Image with chemiluminescent imaging system Perform densitometry and analysis
Product Features	Easy to use No specialized equipment needed Compatible with nearly any liquid sample Proven technology (many publications) Highly sensitive (pg/ml) Sandwich ELISA specificity Higher density than ELISA, Western blot or bead-based multiplex

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