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Mouse Cytokine Array Q4000

ArtNr QAM-CAA-4000-4
Hersteller Raybiotech
Menge 50 Sample Kit
Quantity options 8 Sample Kit 22 Sample Kit 50 Sample Kit
Kategorie
Typ Array
Specific against Mouse (Murine, Mus musculus)
Citations 1. Mao Y., Yen H., Sun Y., Lv Z., Huang R. Development of non-overlapping multiplex antibody arrays for the quantitative measurement of 400 human and 200 mouse proteins in parallel (TECH1P.849). The Journal of Immunology May 1, 2014 vol. 192 no. 1 Supplement 69.17
ECLASS 10.1 32161000
ECLASS 11.0 32161000
UNSPSC 41116126
Similar products EGF, CRP, ACE, Adiponectin, Galectin-3, TPO, Artemin, IFN-gamma, IGFBP-5, IL-10, IL-1 alpha, IL-1 beta, IL-6, Leptin, MMP-2, MMP-3, Osteoprotegerin, Osteopontin, TSLP, VCAM-1, VEGFR1, VEGFR2, HAI-1, SCF, IL-13, IL-4, Resistin, Fractalkine, G-CSF, HGF, IGFBP-2, IL-17, IL-23, TRAIL, VEGF-A, IL-2, CD40, RAGE, Epiregulin, Prolactin, Eotaxin, TACI, IL-21, TIM-1, Clusterin, IL-33, ANGPTL3, BTC, CCL28, CD27, E-Cadherin, IGF-1, Axl, RANTES, CD40 Ligand, P-Cadherin, EDAR, Endoglin, Granzyme B, Galectin-7, NOV, CD48, M-CSF, MIP-1 alpha, GM-CSF, IL-5, IL-7, IL-15, IL-22, IL-9, MCP-1, MadCAM-1, Activin A, Chemerin, CXCL16, Epigen, ICAM-1, IL-3, IL-17E, IL-17F, IL-20, Lymphotactin, MDC, MIG, OX40 Ligand, Persephin, TARC, TECK, VEGF-B, SDF-1 alpha, KC, LIX, PDGF-AA, PF-4, Eotaxin-2, 4-1BB, IL-17B, Limitin, CTLA-4, I-TAC, VEGF-D, IL-12 p70, MIP-2, MCP-5, DLL4, TREML1, Galectin-1, Neprilysin, CD30, E-selectin, VEGFR3, Periostin, Prostasin, Marapsin, Chordin, LOX-1, CD6, Cystatin C, C5a, BAFF R, TGF beta 1, PlGF-2, IL-12 p40, MIP-1 gamma, BLC, MIP-3 alpha, MIP-3 beta, TNF alpha, IGFBP-3, Lipocalin-2, Fetuin A, Fas Ligand, MMP-10, P-selectin, Progranulin, Decorin, Gremlin 1, L-Selectin, Leptin R, Pentraxin 3, B7-1, CT-1, gp130, TREM-1, GITR, ALK-1, MFG-E8, DAN, TROY, Dtk, CXCL15, TNFRI, JAM-A, CCL6, TNFRII, CD30 Ligand, Meteorin, Tryptase epsilon, TWEAK R, Testican 3, Sonic Hedgehog N-Terminal, sFRP-3, Renin 1, Pro-MMP-9, Nope, MBL-2, Kremen-1, Osteoactivin, IL-7 R alpha, IL-3 R beta, IL-28, IL-2 R alpha, IL-17B R, IL-1 ra, IL-1 R4, IGFBP-6, IFN-gamma R1, H60, GITR Ligand, Gas 6, Gas 1, MIP-1 beta (CCL4), Endocan, TCK-1, TRANCE (TNFSF11), CD27 Ligand, TCA-3, HGF R, Dkk-1, Fcg RIIB, Flt-3 L, TWEAK (TNFSF12), ADAMTS1, Fas (TNFRSF6), 6Ckine (CCL21), ANGPTL1 (ANG-3), CD36 (SR-B3), SLAM (CD150), beta FGF
Versandbedingung Gekühlt
Lieferbar
Number of Targets Detected
200
Compatible Sample Types
Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates
Design Principle
Sandwich-based
Solid Support
Glass Slide
Kit Components
  • Mouse Cytokine Array Q4000 Slide(s)
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Lyophilized Standard Mix
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated Fluor
  • 30 ml Centrifuge Tube
  • Adhesive Plastic Strips
  • Manual
Other Materials Required
  • Distilled or deionized water
  • Small plastic boxes or containers
  • Pipettors, pipette tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Aluminum foil
  • Gene microarray scanner or similar laser fluorescence scanner
Protocol Outline
  1. Dry the glass slide
  2. Prepare Standards
  3. Block array surface
  4. Incubate with Samples and Standards
  5. Incubate with Biotinylated Detection Antibody Cocktail
  6. Incubate with Streptavidin-Conjugated Fluor
  7. Disassemble the glass slide
  8. Scan with a gene microarray laser scanner
  9. Perform densitometry and analysis
Detection Method
Fluorescence Laser Scanner
Features
  • More cost-effective than traditional ELISA
  • High specificity and system reproducibility
  • Suitable for diverse sample types
  • Low sample volume requirement: 50 µ, L or less
  • Get results same day (6-hour processing time)
  • Well-suited for high throughput assays
  • Q Analyzer software provides one-step computation
Result Output
Quantitative
Short description
Quantibody Mouse Cytokine Array 4000 Kit. A combination of Mouse Cytokine Array Q4, Mouse Cytokine Array Q5, Mouse Cytokine Array Q6, Mouse Cytokine Array Q7, and Mouse Cytokine Array Q8. Detects 200 Mouse Cytokines. Suitable for all liquid sample types.
Storage
For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s), standard mix, detection antibody cocktail, and Cy3-Conjugated Streptavidin at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
Targets Detected
4-1BB, 6Ckine, ACE, ACRP30, Activin A, ADAMTS1, Adiponectin, ALK-1, Angiopoietin-3, ANGPTL1, ANGPTL3, Apo-1, Artemin, AXL, B7-1, BAFF R, Betacellulin, bFGF, BLC, BTC, C-Reactive Protein, C5a, Cardiotrophin-1, CCL1, CCL11, CCL17, CCL19, CCL2, CCL20, CCL21, CCL22, CCL24, CCL28, CCL3, CCL4, CCL5, CCL6, CCN3, CD105, CD106, CD150, CD152, CD27, CD27 Ligand, CD30, CD30 Ligand, CD32b, CD36, CD40, CD40 Ligand, CD48, CD54, CD6, CD62L, CD80, Chemerin, Chordin, Clusterin, CRP, CT-1, CTLA-4, CX3CL1, CXCL1, CXCL11, CXCL12 alpha, CXCL13, CXCL14, CXCL15, CXCL16, CXCL4, CXCL9, Cystatin C, DAN, Decorin, DKK-1, DLL4, Dtk, E-Cadherin, E-Selectin, EDAR, EGF, Endocan, Endoglin, Eotaxin-1, Eotaxin-2, Epigen, Epiregulin, Fas, Fas Ligand, Fc gamma RIIB, Fetuin A, Flt-3 Ligand, Fractalkine, G-CSF, Galectin-1, Galectin-3, Galectin-7, Gas 1, Gas 6, GITR, GITR Ligand, GM-CSF, gp130, GPNMB, Granzyme B, Gremlin, Gremlin 1, H60, HAI-1, HGF, HGFR, I-309, I-TAC, ICAM-1, IFN-gamma, IFN-gamma R1, IFN-lambda, IGF-1, IGFBP-2, IGFBP-3, IGFBP-5, IGFBP-6, IL-1 alpha, IL-1 beta, IL-1 F1, IL-1 F11, IL-1 F2, IL-1 F3, IL-1 R4, IL-1 Ra, IL-10, IL-12 p40, IL-12 p70, IL-13, IL-15, IL-17, IL-17B, IL-17B R, IL-17C, IL-17E, IL-17F, IL-2, IL-2 R alpha, IL-20, IL-21, IL-22, IL-23, IL-28, IL-3, IL-3 R beta, IL-33, IL-4, IL-5, IL-6, IL-7, IL-7 R alpha, IL-9, JAM-A, KC, KIM-1, Kremen-1, L-Selectin, Leptin, Leptin R, Limitin, Lipocalin-2, LIX, LOX-1, Lungkine, Lymphotactin, M-CSF, MAdCAM-1, Marapsin, MBL-2, MCP-1, MCP-5, MDC, MEC, Meteorin, MFG-E8, MIG, MIP-1 alpha, MIP-1 beta, MIP-1 gamma, MIP-2, MIP-3 alpha, MIP-3 beta, MMP-10, MMP-2, MMP-3, MPIF-2, Neprilysin, NGAL, Nope, NOV, Osteoactivin, Osteopontin, Osteoprotegerin, OX40 Ligand, P-Cadherin, P-Selectin, PDGF-AA, Pentraxin-3, Periostin, Persephin, Platelet Factor 4, PlGF-2, Pro-MMP-9, Progranulin, Prolactin, Prostasin, RAGE, RANTES, Renin 1, Resistin, SCF, SDF-1 alpha, sFRP-3, Shh-N, SLAM, Sonic Hedgehog N-Terminal, SPP1, SR-B3, TACI, TARC, TCA-3, TCK-1, TECK, Testican 3, TGF beta 1, Thrombopoietin, TIM-1, TNF alpha, TNF RI, TNF RII, TNFRSF11B, TNFRSF12, TNFRSF13B, TNFRSF18, TNFRSF19, TNFRSF1A, TNFRSF1B, TNFRSF5, TNFRSF6, TNFRSF7, TNFRSF8, TNFRSF9, TNFSF10, TNFSF11, TNFSF12, TNFSF18, TNFSF4, TNFSF5, TNFSF6, TNFSF7, TNFSF8, TPO, TRAIL, TRANCE, TREM-1, TREML1, TROY, Tryptase epsilon, TSG-14, TSLP, TWEAK, TWEAK R, VCAM-1, VEGF-A, VEGF-B, VEGF-D, VEGF-R2, VEGFR1, VEGFR3, XCL1

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Menge: 50 Sample Kit
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