ArtNr |
QAH-TH17-1-2 |
Hersteller |
Raybiotech
|
Menge |
22 Sample Kit |
Quantity options |
8 Sample Kit
22 Sample Kit
50 Sample Kit
|
Kategorie |
|
Typ |
Array |
Specific against |
Human |
Host |
Hamster - Armenian |
Citations |
1. Celis R., et al. Synovial cytokine expression in psoriatic arthritis and associations with lymphoid neogenesis and clinical features. Celis et al. Arthritis Research & Therapy 2012, 14:R93 |
ECLASS 10.1 |
32161000 |
ECLASS 11.0 |
32161000 |
UNSPSC |
41116126 |
Similar products |
IFN-gamma, IL-10, IL-1 beta, IL-6, IL-13, IL-4, IL-6, IL-23, IL-2, IL-21, IL-17A, GM-CSF, IL-5, IL-22, IL-17F, IL-12 p70, TGF beta 1, TNF beta, MIP-3 alpha, TNF alpha, IL-28 |
Lieferbar |
|
Compatible Sample Types |
Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates |
Design Principle |
Sandwich-based |
Detection Method |
Fluorescence Laser Scanner |
Features |
- More cost-effective than traditional ELISA
- High specificity and system reproducibility
- Suitable for diverse sample types
- Low sample volume requirement: 50 uL or less
- Get results same day (6-hour processing time)
- Well-suited for high throughput assays
- Q Analyzer software provides one-step computation
|
Kit Components |
- Human Th1/Th2/Th17 Array Q1 Slide(s)
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Lyophilized Standard Mix
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated Fluor
- 30 ml Centrifuge Tube
- Adhesive Plastic Strips
- Manual
|
Number of Targets Detected |
20 |
Other Materials Required |
- Distilled or deionized water
- Small plastic boxes or containers
- Pipettors, pipette tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Aluminum foil
- Gene microarray scanner or similar laser fluorescence scanner
|
Protocol Outline |
- Dry the glass slide
- Prepare Standards
- Block array surface
- Incubate with Samples and Standards
- Incubate with Biotinylated Detection Antibody Cocktail
- Incubate with Streptavidin-Conjugated Fluor
- Disassemble the glass slide
- Scan with a gene microarray laser scanner
- Perform densitometry and analysis
|
Result Output |
Quantitative |
Short description |
Quantibody Human Th17 Array 1 Kit. Detects 20 Human Th1, Th2, and Th17 Cytokines. Suitable for all liquid sample types. |
Solid Support |
Glass Slide |
Storage |
For best results, store the entire kit frozen at -20C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s), standard mix, detection antibody cocktail, and Cy3-Conjugated Streptavidin at -20C and all other reagents undiluted at 4C for no more than 3 months. |
Targets Detected |
CCL20, GM-CSF, IFN-gamma, IFN-lambda, IL-1 beta, IL-1 F2, IL-10, IL-12 p70, IL-13, IL-17A, IL-17C, IL-17F, IL-2, IL-21, IL-22, IL-23, IL-28, IL-4, IL-5, IL-6, MIP-3 alpha, TGF beta 1, TNF alpha, TNF beta, TNFSF1B |
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