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Human Cytokine Array Q1

ArtNr QAH-CYT-1-4
Hersteller Raybiotech
Menge 50 Sample Kit
Quantity options 8 Sample Kit 22 Sample Kit 50 Sample Kit
Kategorie
Typ Array
Specific against Human (Homo sapiens)
Citations 1. Jiang, Weidong, Ruo-Pan Huang, and Ruochun Huang. "Protein Expression Profiling by Antibody Array Analysis with Use of Dried Blood Spot Samples on Filter Paper." Journal of Immunological Methods. ScienceDirect, 25 Nov. 2013. Web.
2. Wu H., et al. Upregulation of innate immune responses in a T cell/histiocyte-rich large B cell lymphoma patient with significant autoimmune disorders mimicking systemic lupus erythematosus. Annals of Hematology (2014) 93:353-354
ECLASS 10.1 32161000
ECLASS 11.0 32161000
UNSPSC 41116126
Similar products IFN-gamma, IL-10, IL-1 alpha, IL-1 beta, IL-6, MMP-9, IL-13, IL-4, IL-8, VEGF-A, IL-2, RANTES, MIP-1 alpha, GM-CSF, IL-5, MCP-1, MIP-1 beta, GRO, IL-12p70, TNF alpha
Versandbedingung Gekühlt
Lieferbar
Number of Targets Detected
20
Compatible Sample Types
Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates
Design Principle
Sandwich-based
Solid Support
Glass Slide
Kit Components
  • Human Cytokine Array Q1 Slide(s)
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Lyophilized Standard Mix
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated Fluor
  • 30 ml Centrifuge Tube
  • Adhesive Plastic Strips
  • Manual
Other Materials Required
  • Distilled or deionized water
  • Small plastic boxes or containers
  • Pipettors, pipette tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Aluminum foil
  • Gene microarray scanner or similar laser fluorescence scanner
Protocol Outline
  1. Dry the glass slide
  2. Prepare Standards
  3. Block array surface
  4. Incubate with Samples and Standards
  5. Incubate with Biotinylated Detection Antibody Cocktail
  6. Incubate with Streptavidin-Conjugated Fluor
  7. Disassemble the glass slide
  8. Scan with a gene microarray laser scanner
  9. Perform densitometry and analysis
Detection Method
Fluorescence Laser Scanner
Features
  • More cost-effective than traditional ELISA
  • High specificity and system reproducibility
  • Suitable for diverse sample types
  • Low sample volume requirement: 50 uL or less
  • Get results same day (6-hour processing time)
  • Well-suited for high throughput assays
  • Q Analyzer software provides one-step computation
Result Output
Quantitative
Short description
Quantibody Human Cytokine Array 1 Kit. Detects 20 Human Cytokines. Suitable for all liquid sample types.
Storage
For best results, store the entire kit frozen at -20C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s), standard mix, detection antibody cocktail, and Cy3-Conjugated Streptavidin at -20C and all other reagents undiluted at 4C for no more than 3 months.
Targets Detected
CCL2, CCL3, CCL4, CCL5, CXCL8, GM-CSF, GRO, IFN-gamma, IL-1 alpha, IL-1 beta, IL-1 F2, IL-10, IL-12 p70, IL-13, IL-2, IL-4, IL-5, IL-6, IL-8, MCP-1, MIP-1 alpha, MIP-1 beta, MMP-9, RANTES, TNF alpha, VEGF-A

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 50 Sample Kit
Lieferbar: In stock
lieferbar

Lieferung vsl. bis 24.01.2025 

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