ArtNr |
QAH-CYT-1-2 |
Hersteller |
Raybiotech
|
Menge |
22 Sample Kit |
Quantity options |
8 Sample Kit
22 Sample Kit
50 Sample Kit
|
Kategorie |
|
Typ |
Array |
Specific against |
Human (Homo sapiens) |
Host |
Hamster - Armenian |
Citations |
1. Jiang, Weidong, Ruo-Pan Huang, and Ruochun Huang. "Protein Expression Profiling by Antibody Array Analysis with Use of Dried Blood Spot Samples on Filter Paper." Journal of Immunological Methods. ScienceDirect, 25 Nov. 2013. Web. 2. Wu H., et al. Upregulation of innate immune responses in a T cell/histiocyte-rich large B cell lymphoma patient with significant autoimmune disorders mimicking systemic lupus erythematosus. Annals of Hematology (2014) 93:353-354 |
ECLASS 10.1 |
32161000 |
ECLASS 11.0 |
32161000 |
UNSPSC |
41116126 |
Similar products |
IFN-gamma, IL-10, IL-1 alpha, IL-1 beta, IL-6, MMP-9, IL-13, IL-4, IL-8, VEGF-A, IL-2, RANTES, MIP-1 alpha, GM-CSF, IL-5, MCP-1, MIP-1 beta, GRO, IL-12p70, TNF alpha |
Versandbedingung |
Gekühlt |
Lieferbar |
|
Number of Targets Detected |
20 |
Compatible Sample Types |
Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates |
Design Principle |
Sandwich-based |
Solid Support |
Glass Slide |
Kit Components |
- Human Cytokine Array Q1 Slide(s)
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Lyophilized Standard Mix
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated Fluor
- 30 ml Centrifuge Tube
- Adhesive Plastic Strips
- Manual
|
Other Materials Required |
- Distilled or deionized water
- Small plastic boxes or containers
- Pipettors, pipette tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Aluminum foil
- Gene microarray scanner or similar laser fluorescence scanner
|
Protocol Outline |
- Dry the glass slide
- Prepare Standards
- Block array surface
- Incubate with Samples and Standards
- Incubate with Biotinylated Detection Antibody Cocktail
- Incubate with Streptavidin-Conjugated Fluor
- Disassemble the glass slide
- Scan with a gene microarray laser scanner
- Perform densitometry and analysis
|
Detection Method |
Fluorescence Laser Scanner |
Features |
- More cost-effective than traditional ELISA
- High specificity and system reproducibility
- Suitable for diverse sample types
- Low sample volume requirement: 50 uL or less
- Get results same day (6-hour processing time)
- Well-suited for high throughput assays
- Q Analyzer software provides one-step computation
|
Result Output |
Quantitative |
Short description |
Quantibody Human Cytokine Array 1 Kit. Detects 20 Human Cytokines. Suitable for all liquid sample types. |
Storage |
For best results, store the entire kit frozen at -20C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s), standard mix, detection antibody cocktail, and Cy3-Conjugated Streptavidin at -20C and all other reagents undiluted at 4C for no more than 3 months. |
Targets Detected |
CCL2, CCL3, CCL4, CCL5, CXCL8, GM-CSF, GRO, IFN-gamma, IL-1 alpha, IL-1 beta, IL-1 F2, IL-10, IL-12 p70, IL-13, IL-2, IL-4, IL-5, IL-6, IL-8, MCP-1, MIP-1 alpha, MIP-1 beta, MMP-9, RANTES, TNF alpha, VEGF-A |
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