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Human Cytokine Array G4000

ArtNr AAH-CYT-G4000-8
Hersteller Raybiotech
Menge 8 Sample Kit
Quantity options 4 Sample Kit 8 Sample Kit
Kategorie
Typ Array
Specific against Human (Homo sapiens)
Citations 1. Ando, Yuji, Akihito Yamamoto, and Kohki Matsubara. "Stem Cell-conditioned Medium Accelerates Distraction Osteogenesis through Multiple Regenerative Mechanisms." Bone. ScienceDirect, 02 Jan. 2014. Web.
2. Yang C.Q., MCP-1 Stimulates MMP-9 Expression via ERK 1/2 and p38 MAPK Signaling Pathways in Human Aortic Smooth Muscle Cells. Cell Physiol Biochem 2014,34:266-276 DOI: 10.1159/000362997
3. Peltzer J., et al. Heterogeneous functions of Perinatal Mesenchymal Stromal Cells require a pre-selection before their banking for clinical use. Stem Cells and Development, Sept 2014, epud ahead of print. doi:10.1089/scd.2014.0327
ECLASS 10.1 32161000
ECLASS 11.0 32161000
UNSPSC 41116126
Versandbedingung Gekühlt
Lieferbar
Number of Targets Detected
274
Compatible Sample Types
Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates
Design Principle
Sandwich-based
Solid Support
Glass Slide
Kit Components
  • Human Cytokine Antibody Array G4000 Slide(s)
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated Fluor
  • Lysis Buffer
  • Adhesive Plastic Strips
  • 30 ml Centrifuge Tube
  • Manual
Other Materials Required
  • Small plastic boxes or containers
  • Pipettors, pipette tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Aluminum foil
  • Gene microarray scanner or similar laser fluorescence scanner
Protocol Outline
  1. Dry the glass slide
  2. Block array surface
  3. Incubate with Sample
  4. Incubate with Biotinylated Detection Antibody Cocktail
  5. Incubate with Streptavidin-Conjugated Fluor
  6. Disassemble the glass slide
  7. Scan with a gene microarray laser scanner
  8. Perform densitometry and analysis
Detection Method
Fluorescence Laser Scanner
Features
  • High sensitivity and specificity
  • Low sample volume (10-100 ul per array)
  • Large dynamic range of detection
  • Compatible with most sample types
  • Test 4 or 8 samples on each slide
  • Suitable for high-throughput assays
Result Output
Semi-Quantitative
Short description
RayBio G-Series Human Cytokine Antibody Array 4000 Kit. A combination of Human Cytokine Antibody Array G6, G7, G8, G9, & G10. Detects 274 Human Cytokines. Suitable for all liquid sample types.
Storage
For best results, store the entire kit frozen at -20C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s) and 1X Blocking Buffer at -20C and all other reagents undiluted at 4C for no more than 3 months.
Targets Detected
4-1BB, 6Ckine, ACE-2, AcPL, ACRP30, Activin A, Adiponectin, Adipsin, AgRP, ALCAM, Alpha-fetoprotein, Amphiregulin, Angiogenin, Angiopoietin-1, Angiopoietin-2, Angiostatin, ANGPTL4, Apo-1, AXL, B7-1, BCAM, BCMA, BDNF, beta IG-H3, Beta-2 Microglobulin, beta-NGF, Betacellulin, bFGF, BLC, BMP-4, BMP-5, BMP-6, BMP-7, BTC, c-kit, C-Reactive Protein, CA125, CA15-3, CA19-9, Carbonic Anhydrase IX, Cardiotrophin-1, Cathepsin S, CCL1, CCL11, CCL13, CCL14a, CCL15, CCL16, CCL17, CCL18, CCL19, CCL2, CCL20, CCL21, CCL22, CCL23, CCL24, CCL25, CCL26, CCL27, CCL28, CCL3, CCL4, CCL5, CCL7, CCL8, CD102, CD105, CD106, CD117, CD122, CD14, CD170, CD23, CD26, CD30, CD31, CD32B/C, CD40, CD40 Ligand, CD50, CD54, CD56, CD62L, CD80, CEA, CEACAM-1, CK beta 8-1, CNTF, Common gamma Chain, Cripto, Cripto-1, CRP, CT-1, CTACK, CX3CL1, CXCL1, CXCL10, CXCL11, CXCL12 alpha, CXCL12 beta, CXCL13, CXCL14, CXCL16, CXCL4, CXCL5, CXCL6, CXCL7, CXCL8, CXCL9, DAN, Decorin, DKK-1, DKK-3, DKK-4, DPPIV, DR5, DR6, Dtk, E-Cadherin, E-Selectin, EDA-A2, EG-VEGF, EGF, EGFR, ENA-78, Endoglin, Eotaxin-1, Eotaxin-2, Eotaxin-3, EpCAM, ErbB1, ErbB2, ErbB3, Erythropoietin R, Fas, Fas Ligand, Fc gamma RIIB/C, Ferritin, FGF-4, FGF-6, FGF-7, FGF-9, FLRG, Flt-3 Ligand, Follistatin, Fractalkine, FSH, Furin, G-CSF, Galectin-7, GCP-2, GDF-15, GDNF, GITR, GITR Ligand, GM-CSF, gp130, GRO, GRO alpha, Growth Hormone, HB-EGF, HCC-4, hCG intact, HGF, HVEM, I-309, I-TAC, ICAM-1, ICAM-2, ICAM-3, IFN-gamma, IGF-1, IGF-1 R, IGF-2, IGFBP-1, IGFBP-2, IGFBP-3, IGFBP-4, IGFBP-6, IL-1 alpha, IL-1 beta, IL-1 F1, IL-1 F2, IL-1 F3, IL-1 R1, IL-1 R2, IL-1 R4, IL-1 Ra, IL-10, IL-10 R alpha, IL-10 R beta, IL-11, IL-12 p40, IL-12 p70, IL-13, IL-13 R alpha 2, IL-13 R1, IL-15, IL-16, IL-17, IL-17B, IL-17C, IL-17F, IL-17R, IL-18 BP alpha, IL-18 R beta, IL-2, IL-2 R alpha, IL-2 R beta, IL-2 R gamma, IL-21, IL-21 R, IL-22, IL-28A, IL-29, IL-3, IL-31, IL-4, IL-5, IL-5 R alpha, IL-6, IL-6 R, IL-7, IL-8, IL-9, Insulin, IP-10, KGF, KIM-1, L-Selectin, LAP/TGF beta 1, Leptin, Leptin R, LIF, LIGHT, LIMPII, Luteinizing hormone, Lymphotactin, LYVE-1, M-CSF, M-CSF R, Marapsin, MARC, MCP-1, MCP-2, MCP-3, MCP-4, MDC, MEC, MICA, MICB, MIF, MIG, MIP-1 alpha, MIP-1 beta, MIP-1 delta, MIP-3 alpha, MIP-3 beta, MMP-1, MMP-10, MMP-13, MMP-2, MMP-3, MMP-7, MMP-8, MMP-9, MPIF-1, MPIF-2, MSP alpha/beta, NAP-2, NCAM-1, NGFR, Nidogen-1, NrCAM, NRG1-beta 1, NT-3, NT-4, Oncostatin M, Osteopontin, Osteoprotegerin, PAI-1, PARC, PDGF R alpha, PDGF R beta, PDGF-AA, PDGF-AB, PDGF-BB, PECAM-1, PK1, Platelet Factor 4, PlGF, PPBP, Procalcitonin, Prolactin, PSA-free, PSA-total, RAGE, RANK, RANTES, Resistin, S100 B, SAA, SCF, SCF R, SDF-1 alpha, SDF-1 beta, Serum Amyloid A, Shh-N, Siglec-5, Siglec-9, Sonic Hedgehog N-Terminal, SPP1, ST2, TACE, TARC, TCA-3, TECK, TGF alpha, TGF beta 1, TGF beta 2, TGF beta 3, Thrombopoietin, Thyroglobulin, Tie-1, Tie-2, TIM-1, TIMP-1, TIMP-2, TIMP-4, TNF alpha, TNF beta, TNF RI, TNF RII, TNFRSF10B, TNFRSF10D, TNFRSF11A, TNFRSF11B, TNFRSF14, TNFRSF16, TNFRSF18, TNFRSF21, TNFRSF5, TNFRSF6, TNFRSF8, TNFRSF9, TNFSF14, TNFSF18, TNFSF1B, TNFSF5, TNFSF6, TPO, TRAIL R2, TRAIL R3, TRAIL R4, Trappin-2, TREM-1, TROP1, TSH, TSLP, Ubiquitin+1, uPAR, VCAM-1, VE-Cadherin, VEGF-A, VEGF-C, VEGF-D, VEGFR2, VEGFR3, XCL1, XEDAR

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 8 Sample Kit
Lieferbar: In stock
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