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Human Cytokine Array G3

Human Cytokine Array G3

ArtNr	AAH-CYT-G3-8
Hersteller	Raybiotech
Menge	8 Sample Kit
Quantity options	4 Sample Kit 8 Sample Kit
Kategorie	Oncology Neuroscience Neuroinflammation Metabolism Diabetes Glucose Homeostasis Cholesterol & Lipid Metabolism Regulation of Appetite Neural Signaling By Cell Type Carcinoma Hematology Leukemia Cell Biology Cell Movement Cell Adhesion Obesity Cell Culture Cytokines Cell Status Necrosis Adenocarcinoma Mesothelioma Endocrine Regulation of Lipid Metabolism Arrays By Organ Lung Cancer Bladder Cancer Colorectal Cancer Breast Cancer Testicular & Prostate Cancer Lymphatic System Cancer Antibody Arrays
Typ	Array
Specific against	Human
Host	Hamster - Armenian
Citations	1. Engert S, Rieger L, Kapp M, Becker JC, Dietl J, Kammerer U. Profiling Chemokines, Cytokines and Growth Factors in Human Early Pregnancy Decidua by Protein Array. Am J Reprod Immunol. 2007, 58:129–137. 2. Vasquez-martin A, Colomer R, Menendez JA. Protein array technology to detect HER2 (erbB-2)-induced 'cytokine signature' in breast cancer. Eur J Cancer. 2007,43:1117–1124. 3. Wang S, Yang J, Qian J, Wezeman M, Kwak LW, Yi Q. Tumor evasion of the immune system: inhibiting p38 MAPK signaling restores the function of dendritic cells in multiple myeloma. Blood. 2006,107:2431-2439. 4. Eldeen M B,Deshmane S L,Simbiri K, Khalili K, Amini S,et al.MH2 domain of Smad3 reduces HIV-1 Tat-induction of cytokine secretion. J Neuroimmunol. 2006,176:174 – 180 5. Gerrits J H,Wetering J,Weimar W,Besouw N M. Donor-Reactive Cytokine Production After HLA-Identical Living Related Kidney Transplantation: A Protein-Array Analysis.Transplantation Proceedings,2006,38, 2825–2827 6. Rethi B, Gogolak P, Szatmari I, Veres A, et al. SLAM/SLAM interactions inhibit CD40-induced production of inflammatory cytokines in monocyte-derived dendritic cells. Blood. 2006,107:2821-2829. 7. Li J,Bentsman G,Potash M,Volsky D.Human immunodeficiency virus type 1 efficiently binds to human fetal astrocytes and induces neuroinflammatory responses independent of infection.BMC Neuroscience.2007, 8:31 8. Fiorucci M,Boulant S, Fournillier A, Abraham J, Lavergne J,et al.Expression of the alternative reading frame protein of Hepatitis C virus induces cytokines involved in hepatic injuries.J Gen Virol. 2007,88:1149–1162 9. Potian JA, Aviv H, Ponzio NM, Harrison JS, Rameshwar P. Veto-Like Activity of Mesenchymal Stem Cells: Functional Discrimination Between Cellular Responses to Alloantigens and Recall Antigens. J Immunol. 2003,17(1)3426.3434. 10. Julia P, Daniel S.Effect of gonococcal lipooligosaccharide variation on human monocytic cytokine profile.BMC Microbiology. 2007,7:7 11. Abbott DW, Yang Y, Hutti JE, Madhavarapu S, Kelliher MA, Cantley LC. Coordinated Regulation of Toll-Like Receptor and NOD2 Signaling by K63-Linked Polyubiquitin Chains. Mol Cell Biol. 2007,27(17):6012-6025. 12. Benitez S, Camacho M, Bancells C, Vila L, Sanchez-Quesada JL, Ordonez-Llanos J. Wide proinflammatory effect of electronegative low-density lipoprotein on human endothelial cells assayed by a protein array. Biochim Biophys Acta. 2006,1761:1014-1021. 13. Patrone JB, Bish SE, Stein DC. TNF-alpha-Independent IL-8 Expression: Alterations in Bacterial Challenge Dose Cause Differential Human Monocytic Cytokine Response. J Immunol. 2006,177:1314-1322. 14. Gogolak P, Rethi B, Szatmari I, Lanyi A, et al. Differentiation of CD1a- and CD1a+ monocyte-derived dendritic cells is biased by lipid environment and PPAR-gamma. Blood. 2007, 109:643-652. 15. Chiu TT, Leung WY, Moyer MP, Strieter RM, Rozengurt E. Protein kinase D2 mediates lysophosphatidic acid-induced interleukin 8 production in nontransformed human colonic epithelial cells through NF-kB. Am J Physiol Cell Physiol.2007,292:C767–C777. 16. Robson RL, Reed NA, Horvat RT. Differential activation of inflammatory pathways in A549 type II pneumocytes by Streptococcus pneumoniae strains with different adherence properties. BMC Infect Dis. 2006,6:71. Available at http://www.biomedcentral.com/1471-2334/6/71. Last accessed Jan 25, 2008. 17. Bogdanski D, Esenwein SA, Prymak O, Epple M, Muhr G, Koller M. Release of Leucocyte Mediators after Contact to Coated and Non-coated NITI-SMA. Biomedizinische Technik Jg. 2004,49:562-563. 18. Ritter M, Mennerich D, Weith A, Seither P. Characterization of Toll-like receptors in primary lung epithelial cells: strong impact of the TLR3 ligand poly(I:C) on the regulation of Toll-like receptors, adaptor proteins and inflammatory response. J Inflamm. 2005, 2:16. Available at: http://www.journal-inflammation.com/content/2/1/16. Last accessed Jan 25, 2008. 19. Tay SS, McCormack A, Rose ML. Effect of Cognate Human CD4+ T Cell and Endothelial Cell Interactions Upon Chemokine Production. Transplant. 2004,78(7):987-994. 20. Duenas AI, Aceves M, Orduna A, Diaz R, Crespo MS, Garcia-Rodriguez C. Francisella tularensis LPS induces the production of cytokines in human monocytes and signals via Toll-like receptor 4 with much lower potency than E. coli LPS. Intl Immunol. 2006,18(5):785-795. 21. Marks DJB, Harbord MWN, MacAllister R, Rahman FZ, Young J, et al. Defective acute inflammation in Crohn's disease: a clinical investigation. Lancet. 2006,367:668-678. 22. Kaplin AI, Deshpande DM, Scott E, Krishnan C, Carmen JS, et al. IL-6 induces regionally selective spinal cord injury in patients with the neuroinflammatory disorder transverse myelitis. J Clin Invest. 2005,115:2731-2741. 23. Nichols MT, Gidey E, Matzakos T, Dahl R, et al. Secretion of Cytokines and Growth Factors Into Autosomal Dominant Polycystic Kidney Disease Liver Cyst Fluid. Hepatology. 2004,40:836-846. 24. Coppinger JA, O'Connor R, Wynne K, Flanagan M, Sullivan M, et al. Moderation of the platelet releasate response by aspirin. Blood. 2007,109:4786-4792. 25. Bailey L, Kuroyanagi Y, Franco-Penteado CF, Conran N, Costa FF, et al. Expression of the gamma-globulin gene is sustained by the cAMP-dependent pathway in beta-thalassaemia. Br J Haematol. 2007,138:382–395. 26. Vasquez-martin A, Colomer R, Menendez JA. Protein array technology to detect HER2 (erbB-2)-induced 'cytokine signature' in breast cancer. Eur J Cancer. 2007,43:1117–1124. 27. Patrone J, Bish S, Stein D. TNF-alpha -Independent IL-8 Expression: Alterations in Bacterial Challenge Dose Cause Differential Human Monocytic Cytokine Response.J Immunol. 2006,177:1314–1322. 28. De Ceuninck F, Marcheteau E, Berger S, Caliez A, et al. Assessment of Some Tools for the Characterization of the Human Osteoarthritic Cartilage Proteome. J Biomol Tech. 2005,16:256–265.
ECLASS 10.1	32161000
ECLASS 11.0	32161000
UNSPSC	41116126
Similar products	EGF, IFN-gamma, IL-10, IL-1 alpha, IL-1 beta, IL-6, Leptin, SCF, IL-13, IL-4, IL-8, IL-6, SDF-1, G-CSF, VEGF-A, IL-2, IGF-1, RANTES, M-CSF, MCP-3, GM-CSF, IL-5, IL-7, IL-15, MCP-1, ENA-78, IL-3, MDC, MIG, Oncostatin M, TARC, PDGF-BB, MCP-2, GRO, I-309, MIP-1 delta, TGF beta 1, TNF beta, Thrombopoietin, TNF alpha, Angiogenin, IL-12 p40/p70, GRO alpha
Lieferbar
Compatible Sample Types	Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates
Design Principle	Sandwich-based
Detection Method	Fluorescence Laser Scanner
Features	High sensitivity and specificity Low sample volume (10-100 ul per array) Large dynamic range of detection Compatible with most sample types Test 4 or 8 samples on each slide Suitable for high-throughput assays
Kit Components	Human Cytokine Antibody Array G3 Slide(s) Blocking Buffer Wash Buffer 1 Wash Buffer 2 Biotinylated Detection Antibody Cocktail Streptavidin-Conjugated Fluor Lysis Buffer Adhesive Plastic Strips 30 ml Centrifuge Tube Manual
Number of Targets Detected	42
Other Materials Required	Small plastic boxes or containers Pipettors, pipette tips and other common lab consumables Orbital shaker or oscillating rocker Aluminum foil Gene microarray scanner or similar laser fluorescence scanner
Protocol Outline	Dry the glass slide Block array surface Incubate with Sample Incubate with Biotinylated Detection Antibody Cocktail Incubate with Streptavidin-Conjugated Fluor Disassemble the glass slide Scan with a gene microarray laser scanner Perform densitometry and analysis
Result Output	Semi-Quantitative
Short description	RayBio G-Series Human Cytokine Antibody Array 3 Kit. Detects 42 Humans Cytokines. Suitable for all liquid sample types.
Solid Support	Glass Slide
Storage	For best results, store the entire kit frozen at -20C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s) and 1X Blocking Buffer at -20C and all other reagents undiluted at 4C for no more than 3 months.
Targets Detected	Angiogenin, CCL1, CCL15, CCL17, CCL2, CCL22, CCL5, CCL7, CCL8, CXCL1, CXCL12 alpha, CXCL8, CXCL9, EGF, ENA-78, G-CSF, GM-CSF, GRO, GRO alpha, I-309, IFN-gamma, IGF-1, IL-1 alpha, IL-1 beta, IL-1 F1, IL-1 F2, IL-10, IL-12 p40/p70, IL-13, IL-15, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, Leptin, M-CSF, MARC, MCP-1, MCP-2, MCP-3, MDC, MIG, MIP-1 delta, Oncostatin M, PDGF-BB, RANTES, SCF, SDF-1 alpha, TARC, TCA-3, TGF beta 1, Thrombopoietin, TNF alpha, TNF beta, TNFSF1B, TPO, VEGF-A

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