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Human Cytokine Array C4000

ArtNr AAH-CYT-4000-4
Hersteller Raybiotech
Menge 4 Sample Kit
Quantity options 2 Sample Kit 4 Sample Kit 8 Sample Kit
Kategorie
Typ Array
Specific against Human (Homo sapiens)
Citations Takahashi, Kazue, Patience Moyo, and Lorencia Chigweshe. "Efficacy of Recombinant Chimeric Lectins, Consisting of Mannose Binding Lectin and L-ficolin, against Influenza A Viral Infection in Mouse Model Study." Virus Research. ScienceDirect, 26 Dec. 2013. Web.
ECLASS 10.1 32161000
ECLASS 11.0 32161000
UNSPSC 41116126
Versandbedingung Gekühlt
Lieferbar
Number of Targets Detected
274
Compatible Sample Types
Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates
Design Principle
Sandwich-based
Solid Support
Membrane
Kit Components
  • Human Cytokine Antibody Array C4000 Membranes
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated HRP
  • Detection Buffer C
  • Detection Buffer D
  • Lysis Buffer
  • 8-Well Incubation Tray
  • Plastic Sheets
  • Array Templates
  • Manual
Other Materials Required
  • Pipettors, pipet tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Tissue Paper, blotting paper or chromatography paper
  • Adhesive tape or Saran Wrap
  • Distilled or de-ionized water
  • A chemiluminescent blot documentation system (such as UVPs ChemiDoc-It or EpiChem II Benchtop Darkroom), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline
  1. Block membranes
  2. Incubate with Sample
  3. Incubate with Biotinylated Detection Antibody Cocktail
  4. Incubate with HRP-Conjugated Streptavidin
  5. Incubate with Detection Buffers
  6. Image with chemiluminescent imaging system
  7. Perform densitometry and analysis
Detection Method
Chemiluminescence
Features
  • Easy to use
  • No specialized equipment needed
  • Compatible with nearly any liquid sample
  • Proven technology (many publications)
  • Highly sensitive (pg/ml)
  • Sandwich ELISA specificity
  • Higher density than ELISA, Western blot or bead-based multiplex
Result Output
Semi-Quantitative
Short description
RayBio C-Series Human Cytokine Antibody Array 4000 Kit. A combination of Human Cytokine Antibody Array C6, C7, C8, C9, & C10. Detects 274 Human Cytokines. Suitable for all liquid sample types.
Storage
For best results, store the entire kit frozen at -20C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20C and all other reagents undiluted at 4C for no more than 3 months.
Targets Detected
4-1BB, 6Ckine, ACE-2, AcPL, ACRP30, Activin A, Adiponectin, Adipsin, AgRP, ALCAM, Alpha-fetoprotein, Amphiregulin, Angiogenin, Angiopoietin-1, Angiopoietin-2, Angiostatin, ANGPTL4, Apo-1, AXL, B7-1, BCAM, BCMA, BDNF, beta IG-H3, Beta-2 Microglobulin, beta-NGF, Betacellulin, bFGF, BLC, BMP-4, BMP-5, BMP-6, BMP-7, BTC, c-kit, C-Reactive Protein, CA125, CA15-3, CA19-9, Carbonic Anhydrase IX, Cardiotrophin-1, Cathepsin S, CCL1, CCL11, CCL13, CCL14a, CCL15, CCL16, CCL17, CCL18, CCL19, CCL2, CCL20, CCL21, CCL22, CCL23, CCL24, CCL25, CCL26, CCL27, CCL28, CCL3, CCL4, CCL5, CCL7, CCL8, CD102, CD105, CD106, CD117, CD122, CD14, CD170, CD23, CD26, CD30, CD31, CD32B/C, CD40, CD40 Ligand, CD50, CD54, CD56, CD62L, CD80, CEA, CEACAM-1, CK beta 8-1, CNTF, Common gamma Chain, Cripto, Cripto-1, CRP, CT-1, CTACK, CX3CL1, CXCL1, CXCL10, CXCL11, CXCL12 alpha, CXCL12 beta, CXCL13, CXCL14, CXCL16, CXCL4, CXCL5, CXCL6, CXCL7, CXCL8, CXCL9, DAN, Decorin, DKK-1, DKK-3, DKK-4, DPPIV, DR5, DR6, Dtk, E-Cadherin, E-Selectin, EDA-A2, EG-VEGF, EGF, EGFR, ENA-78, Endoglin, Eotaxin-1, Eotaxin-2, Eotaxin-3, EpCAM, ErbB1, ErbB2, ErbB3, Erythropoietin R, Fas, Fas Ligand, Fc gamma RIIB/C, Ferritin, FGF-4, FGF-6, FGF-7, FGF-9, FLRG, Flt-3 Ligand, Follistatin, Fractalkine, FSH, Furin, G-CSF, Galectin-7, GCP-2, GDF-15, GDNF, GITR, GITR Ligand, GM-CSF, gp130, GRO, GRO alpha, Growth Hormone, HB-EGF, HCC-4, hCG intact, HGF, HVEM, I-309, I-TAC, ICAM-1, ICAM-2, ICAM-3, IFN-gamma, IGF-1, IGF-1 R, IGF-2, IGFBP-1, IGFBP-2, IGFBP-3, IGFBP-4, IGFBP-6, IL-1 alpha, IL-1 beta, IL-1 F1, IL-1 F2, IL-1 F3, IL-1 R1, IL-1 R2, IL-1 R4, IL-1 Ra, IL-10, IL-10 R alpha, IL-10 R beta, IL-11, IL-12 p40, IL-12 p70, IL-13, IL-13 R alpha 2, IL-13 R1, IL-15, IL-16, IL-17, IL-17B, IL-17C, IL-17F, IL-17R, IL-18 BP alpha, IL-18 R beta, IL-2, IL-2 R alpha, IL-2 R beta, IL-2 R gamma, IL-21, IL-21 R, IL-22, IL-28A, IL-29, IL-3, IL-31, IL-4, IL-5, IL-5 R alpha, IL-6, IL-6 R, IL-7, IL-8, IL-9, Insulin, IP-10, KGF, KIM-1, L-Selectin, LAP/TGF beta 1, Leptin, Leptin R, LIF, LIGHT, LIMPII, Luteinizing hormone, Lymphotactin, LYVE-1, M-CSF, M-CSF R, Marapsin, MARC, MCP-1, MCP-2, MCP-3, MCP-4, MDC, MEC, MICA, MICB, MIF, MIG, MIP-1 alpha, MIP-1 beta, MIP-1 delta, MIP-3 alpha, MIP-3 beta, MMP-1, MMP-10, MMP-13, MMP-2, MMP-3, MMP-7, MMP-8, MMP-9, MPIF-1, MPIF-2, MSP alpha/beta, NAP-2, NCAM-1, NGFR, Nidogen-1, NrCAM, NRG1-beta 1, NT-3, NT-4, Oncostatin M, Osteopontin, Osteoprotegerin, PAI-1, PARC, PDGF R alpha, PDGF R beta, PDGF-AA, PDGF-AB, PDGF-BB, PECAM-1, PK1, Platelet Factor 4, PlGF, PPBP, Procalcitonin, Prolactin, PSA-free, PSA-total, RAGE, RANK, RANTES, Resistin, S100 B, SAA, SCF, SCF R, SDF-1 alpha, SDF-1 beta, Serum Amyloid A, Shh-N, Siglec-5, Siglec-9, Sonic Hedgehog N-Terminal, SPP1, ST2, TACE, TARC, TCA-3, TECK, TGF alpha, TGF beta 1, TGF beta 2, TGF beta 3, Thrombopoietin, Thyroglobulin, Tie-1, Tie-2, TIM-1, TIMP-1, TIMP-2, TIMP-4, TNF alpha, TNF beta, TNF RI, TNF RII, TNFRSF10B, TNFRSF10D, TNFRSF11A, TNFRSF11B, TNFRSF14, TNFRSF16, TNFRSF18, TNFRSF21, TNFRSF5, TNFRSF6, TNFRSF8, TNFRSF9, TNFSF14, TNFSF18, TNFSF1B, TNFSF5, TNFSF6, TPO, TRAIL R2, TRAIL R3, TRAIL R4, Trappin-2, TREM-1, TROP1, TSH, TSLP, Ubiquitin+1, uPAR, VCAM-1, VE-Cadherin, VEGF-A, VEGF-C, VEGF-D, VEGFR2, VEGFR3, XCL1, XEDAR

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 4 Sample Kit
Lieferbar: In stock
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