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Human Cytokine Array C4

ArtNr AAH-CYT-4-4
Hersteller Raybiotech
Menge 4 Sample Kit
Quantity options 2 Sample Kit 4 Sample Kit 8 Sample Kit
Kategorie
Typ Array
Specific against Human (Homo sapiens)
Host Hamster - Armenian
Citations 1. De Ceuninck F, Marcheteau E, Berger S, Caliez A, et al. Assessment of Some Tools for the Characterization of the Human Osteoarthritic Cartilage Proteome. J Biomol Tech. 2005,16:256–265.
ECLASS 10.1 32161000
ECLASS 11.0 32161000
UNSPSC 41116126
Similar products BDNF, Osteoprotegerin, PLGF, TIMP-1, MIF, LIF, PARC, NT-4, TIMP-2, IP-10, Eotaxin-2, Eotaxin-3, MCP-4, NAP-2, Eotaxin-1, FGF-6, FGF-4, TGF beta 2, TGF beta 3, BLC, MIP-3 alpha, NT-3, Light, Ck beta 8-1
Versandbedingung Gekühlt
Lieferbar
Number of Targets Detected
36
Compatible Sample Types
Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates
Design Principle
Sandwich-based
Solid Support
Membrane
Kit Components
  • Human Cytokine Antibody Array C4 Membranes
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated HRP
  • Detection Buffer C
  • Detection Buffer D
  • Lysis Buffer
  • 8-Well Incubation Tray
  • Plastic Sheets
  • Array Templates
  • Manual
Other Materials Required
  • Pipettors, pipet tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Tissue Paper, blotting paper or chromatography paper
  • Adhesive tape or Saran Wrap
  • Distilled or de-ionized water
  • A chemiluminescent blot documentation system (such as UVPs ChemiDoc-It or EpiChem II Benchtop Darkroom), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline
  1. Block membranes
  2. Incubate with Sample
  3. Incubate with Biotinylated Detection Antibody Cocktail
  4. Incubate with HRP-Conjugated Streptavidin
  5. Incubate with Detection Buffers
  6. Image with chemiluminescent imaging system
  7. Perform densitometry and analysis
Detection Method
Chemiluminescence
Features
  • Easy to use
  • No specialized equipment needed
  • Compatible with nearly any liquid sample
  • Proven technology (many publications)
  • Highly sensitive (pg/ml)
  • Sandwich ELISA specificity
  • Higher density than ELISA, Western blot or bead-based multiplex
Result Output
Semi-Quantitative
Short description
RayBio C-Series Human Cytokine Antibody Array 4 Kit. Detects 36 Human Cytokines. Suitable for all liquid sample types.
Storage
For best results, store the entire kit frozen at -20C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20C and all other reagents undiluted at 4C for no more than 3 months.
Targets Detected
BDNF, Eotaxin-3, CCL26, Flt-3 Ligand, IGFBP-1, IP-10, CXCL10, MIP-3 alpha, CCL20, PARC, CCL18, TIMP-2, BLC, CXCL13, FGF-4, Fractalkine, CX3CL1, IGFBP-2, LIF, NAP-2, PPBP, CXCL7, PLGF, Ck beta 8-1, CCL23, FGF-6, GCP-2, CXCL6, IGFBP-3, Light, TNFSF14, NT-3, TGF beta 2, Eotaxin-1, CCL11, FGF-7, KGF, GDNF, IGFBP-4, MCP-4, CCL13, NT-4, TGF beta 3, Eotaxin-2, MPIF-2, CCL24, FGF-9, HGF, IL-16, MIF, Osteoprotegerin, TNFRSF11B, TIMP-1

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 4 Sample Kit
Lieferbar: In stock
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