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Human Cytokine Array C10

Human Cytokine Array C10

ArtNr	AAH-CYT-10-4
Hersteller	Raybiotech
Menge	4 Sample Kit
Quantity options	2 Sample Kit 4 Sample Kit 8 Sample Kit
Kategorie	Oncology Arrays By Organ Lung Cancer Bladder Cancer Colorectal Cancer Breast Cancer Testicular & Prostate Cancer Gallbladder Cancer Gastric Cancer Pancreatic Cancer Thyroid Cancer Hepatic Cancer Uterine, Ovarian & Cervical Cancer Antibody Arrays
Typ	Array
Specific against	Human (Homo sapiens)
Host	Hamster - Armenian
Citations	Kim M, Oskarsson T, Acharyya S, Nguyen D, Zhang X, et al.Tumor Self-Seeding by Circulating Cancer Cells. Cell. 2009,139(7):1315–1326.
ECLASS 10.1	32161000
ECLASS 11.0	32161000
UNSPSC	41116126
Versandbedingung	Gekühlt
Lieferbar
Number of Targets Detected	49
Compatible Sample Types	Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates
Design Principle	Sandwich-based
Solid Support	Membrane
Kit Components	Human Cytokine Antibody Array C10 Membranes Blocking Buffer Wash Buffer 1 Wash Buffer 2 Biotinylated Detection Antibody Cocktail Streptavidin-Conjugated HRP Detection Buffer C Detection Buffer D Lysis Buffer 8-Well Incubation Tray Plastic Sheets Array Templates Manual
Other Materials Required	Pipettors, pipet tips and other common lab consumables Orbital shaker or oscillating rocker Tissue Paper, blotting paper or chromatography paper Adhesive tape or Saran Wrap Distilled or de-ionized water A chemiluminescent blot documentation system (such as UVPs ChemiDoc-It or EpiChem II Benchtop Darkroom), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline	Block membranes Incubate with Sample Incubate with Biotinylated Detection Antibody Cocktail Incubate with HRP-Conjugated Streptavidin Incubate with Detection Buffers Image with chemiluminescent imaging system Perform densitometry and analysis
Detection Method	Chemiluminescence
Features	Easy to use No specialized equipment needed Compatible with nearly any liquid sample Proven technology (many publications) Highly sensitive (pg/ml) Sandwich ELISA specificity Higher density than ELISA, Western blot or bead-based multiplex
Result Output	Semi-Quantitative
Short description	RayBio C-Series Human Cytokine Antibody Array 10 Kit. Detects 49 Human Cytokines. Suitable for all liquid sample types.
Storage	For best results, store the entire kit frozen at -20C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20C and all other reagents undiluted at 4C for no more than 3 months.
Targets Detected	4-1BB, 6Ckine, ACE-2, Alpha-fetoprotein, Angiopoietin-1, Angiostatin, ANGPTL4, BCMA, beta IG-H3, Beta-2 Microglobulin, C-Reactive Protein, CA125, CA15-3, CA19-9, CA9, Carbonic Anhydrase IX, Cathepsin S, CCL14a, CCL21, CD23, CD26, CD40 Ligand, CEA, CEACAM-1, Cripto, Cripto-1, CRP, DAN, Decorin, DKK-1, DKK-3, DKK-4, DPPIV, E-Cadherin, EDA-A2, EG-VEGF, EpCAM, ErbB2, Erythropoietin R, FSH, HB-EGF, hCG intact, HVEM, IL-13 R1, IL-17B, IL-17C, IL-17F, IL-17R, IL-21, PK1, Procalcitonin, PSA-free, S100 B, Shh-N, Sonic Hedgehog N-Terminal, Thyroglobulin, TNFRSF14, TNFRSF17, TNFRSF9, TNFSF5, TROP1, Ubiquitin+1

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