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Human Cytokine Array C1

Human Cytokine Array C1

ArtNr	AAH-CYT-1-4
Hersteller	Raybiotech
Menge	4 Sample Kit
Quantity options	2 Sample Kit 4 Sample Kit 8 Sample Kit
Kategorie	Oncology Neuroscience Neuroinflammation Metabolism Diabetes Glucose Homeostasis Cholesterol & Lipid Metabolism By Cell Type Carcinoma Hematology Leukemia Cell Biology Cell Movement Cell Adhesion Obesity Cell Culture Cytokines Cell Status Necrosis Mesothelioma Arrays By Organ Bladder Cancer Colorectal Cancer Breast Cancer Heart & Blood Cancer Bone Cancer Antibody Arrays
Typ	Array
Specific against	Human
Host	Hamster - Armenian
Citations	1. Modulation of Cytokine Release and Gene Expression by the Immunosuppressive Domain of gp41 of HIV-1 2. Villar CC, Kashleva H, Mitchell AP, Dongari-Bagtzoglou A. Invasive Phenotype of Candida albicans Affects the Host Proinflammatory Response to Infection. Infect Immun. 2005,73:4588-4595. 3. Bohmova K,Hladikova Z,Cerny M , Flajsmanova K, Vrabelova Z,et al. Cord Blood Cytokine Profile Detection in Neonates with T1D Parents–Monitoring of Cellular Auto-reactivity Using Protein Microarray. Scand J Immunol.2007,66:563–571 4. Joner M, Farb A, Cheng Q, Finn A , Acampado E,et al.Pioglitazone Inhibits In-Stent Restenosis in Atherosclerotic Rabbits by Targeting Transforming Growth Factor- and MCP-1.Arterioscler Thromb Vasc Biol. 2007,27:182 5. Gruber H E,Hoelscher G,Ingram J A, Chow Y,Loeffler B,et al. 1,25(OH)2-Vitamin D3 Inhibits Proliferation and Decreases Production of Monocyte Chemoattractant Protein-1, rombopoietin, VEGF, and Angiogenin by Human Annulus Cells In Vitro. Spine. 2008,33 755-765 6. Liao H, Su S,Chen Y,Chou C, Kuo C.Norcantharidin preferentially induces apoptosis in human leukemic Jurkat cells without affecting viability of normal blood mononuclear cells. Food Chem Toxicol.2007,45 :1678–1687 7. Pulai JI, Chen H, Im H-J. Kumar S, et al. NF-kappaB Mediates the Stimulation of Cytokine and Chemokine Expression by Human Articular Chondrocytes in Response to Fibronectin Fragments. J Immunol. 2005, 174:5781-5788. 8. Sze S, Kleijn D, Lai R, Tan E, Zhao H,et al.Elucidating the Secretion Proteome of Human Embryonic Stem Cell-derived Mesenchymal Stem Cells.Mol Cell Proteomics. 2007, 6: 1680-1693 9. Patton GW, Powell DA, Hakki A, Friedman H, Pross S. Nicotine modulation of cytokine induction by LPS-stimulated monocytes and coronary artery endothelial cells. Int Immunopharmacol. 2006, 6:26-35. 10. Li L. Oral epithelium–Candida glabrata interactions in vitro.Oral Microbiol Immun.2007: 22: 182–187 11. Turtinen L W ,Prall D N , Bremer L A ,Nauss R E, Hartsel S C.Antibody Array-Generated Profiles of Cytokine Release from THP-1 Leukemic Monocytes Exposed to Different Amphotericin B Formulations. Antimicrob Agents Chemother. 2004,48(2):396–403. 12. Yen Y-T, Liao F, Hsiao C-H, Kao C-L, Chen Y-C, Wu-Hsieh BA. Modeling the Early Events of Severe Acute Respiratory Syndrome Coronavirus Infection In Vitro. J Virol. 2006,80(6):2684-2693. 13. Villar CC, Kashleva H, Mitchell AP, Dongari-Bagtzoglou A. Invasive Phenotype of Candida albicans Affects the Host Proinflammatory Response to Infection. Infect Immun. 2005,73:4588-4595. 14. Pockaj BA, Basu, GD, Pahtangey LB, Gray RJ, et al. Reduced T-Cell and Dendritic Cell Function Is Related to Cyclooxygenase-2 Overexpression and Prostaglandin E2 Secretion in Patients With Breast Cancer. Annal Surg Oncol. 2004,11(3):328–339. 15. Sohara Y, Shimada H, Minkin C, Erdreich-Epstein A, Nolta J,et al. Bone Marrow Mesenchymal Stem Cells Provide an Alternate Pathway of Osteoclast Activation and Bone Destruction by Cancer Cells. Cancer Res. 2005,65(4):1129–1135 16. Norata GD, Tibolla G, Seccomandi PM, Poletti A, Catapano AL. Dihydrotestosterone Decreases Tumor Necrosis Factor-alpha and Lipoploysaccharide-Induced Inflammatory Response in Human Endothelial Cells. J Clin Endocrinol Metab. 2006,91:546–554. 17. Wang M, Xiao GG, Li N, Xie Y, Loo JA, Nel AE. Use of a fluorescent phosphoprotein dye to characterize oxidative stress-induced signaling pathway components in macrophage and epithelial cultures exposed to diesel exhaust particle chemicals. Electrophoresis. 2005,26:2092–2108. 18. Choi K-S, Park JT, Dumler JS. Anaplasma phagocytophilum Delay of Neutrophil Apoptosis through the p38 Mitogen-Activated Protein Kinase Signal Pathway. Infect. Immun. December 2005 vol. 73 no. 12 8209-8218 19. Fujiwara, H et al. In vitro induction of myeloid leukemia-specific CD4 and CD8 T cells by CD40 ligand-activated B cells gene modified to express primary granule proteins. In vitro induction of myeloid leukemia-specific CD4 and CD8 T cells by CD40 ligand-activated B cells gene modified to express primary granule proteins. In vitro induction of myeloid leukemia-specific CD4 and CD8 T cells by CD40 ligand-activated B cells gene modified to express primary granule proteins. Clin. Cancer Res. Volume 11, Issue 12, Pages 4495-503, Jun 2005 20. Cattaneo V., Tribulatti M., Carabelli J., et al. Galectin-8 elicits pro-inflammatory activities in the endothelium. Glycobiology, Published Online ahead of print, June 22 2014. doi:10.1093/glycob/cwu060 21. Sos M., et al. Oncogene Mimicry as a Mechanism of Primary Resistance to BRAF Inhibitors. Cell Reports Available online 7 August 2014. DOI: 10.1016/j.celrep.2014.07.010 22. Alkhatib B., et al. ACUTE MECHANICAL INJURY OF THE HUMAN INTERVERTEBRAL DISC: LINK TO DEGENERATION AND PAIN. European Cells and Materials, 2014 (28) 98-111. 23. Nagata E, Fujii N, Hosomichi K, Mitsunaga S, Suzuki Y, et al. (2014) Possible Association between Dysfunction of Vitamin D Binding Protein (GC Globulin) and Migraine Attacks. PLoS ONE 9(8): e105319. doi:10.1371/journal.pone.0105319 24. Lee CH., et al. IL-1beta Promotes Malignant Transformation and Tumor Aggressiveness in Oral Cancer. Journal of Cellular Physiology Sept 2014, Epub Ahead of print. DOI: 10.1002/jcp.24816 25. Nazzal D., et al. Human thymus medullary epithelial cells promote regulatory T-cell generation by stimulating interleukin-2 production via ICOS ligand. Cell Death and Disease (2014) 5, e1420, doi:10.1038/cddis.2014.377
ECLASS 10.1	32161000
ECLASS 11.0	32161000
UNSPSC	41116126
Similar products	IFN-gamma, IL-10, IL-1 alpha, IL-6, IL-13, IL-8, IL-6, G-CSF, IL-2, RANTES, MCP-3, GM-CSF, IL-5, IL-7, IL-15, MCP-1, IL-3, MIG, MCP-2, GRO, TGF beta 1, TNF beta, TNF alpha, GRO alpha
Lieferbar
Number of Targets Detected	23
Species Detected	Human
Compatible Sample Types	Cell Culture Supernatants Cell Lysates Plasma Serum Tissue Lysates
Design Principle	Sandwich-based
Method of Detection	Chemiluminescence
Quantitative/Semi-Quantitative	Semi-Quantitative
Solid Support	Membrane
Gene Symbols	CCL2 \| CCL5 \| CCL7 \| CCL8 \| CSF2 \| CSF3 \| CXCL1 \| CXCL8 \| CXCL9 \| IFNG\| IL10 \| IL13 \| IL15 \| IL1A \| IL2 \| IL3 \| IL5 \| IL6 \| IL7 \| LTA \| TGFB1 \| TNF
Application Information	Multiplexed Protein Detection Detection of Relative Protein Expression Detecting Patterns of Cytokine Expression Biomarker/ Key Factor Screening Identifying Key Factors Confirming a Biological Process
Kit Components	Human Cytokine Antibody Array C1 Membranes Blocking Buffer Wash Buffer 1 Wash Buffer 2 Biotinylated Detection Antibody Cocktail Streptavidin- Conjugated H R P Detection Buffer C Detection Buffer D Lysis Buffer8- Well Incubation Tray Plastic Sheets Array Templates Manual
Other Materials Required	Pipettors, pipet tips and other common lab consumables Orbital shaker or oscillating rocker Tissue Paper, blotting paper or chromatography paper Adhesive tape or Saran Wrap Distilled or de-ionized water A chemiluminescent blot documentation system (such as U V P's Chemi Doc- It® or Epi Chem I I Benchtop Darkroom or G E's Image Quant™ L A S 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline	Block membranes Incubate with Sample Incubate with Biotinylated Detection Antibody Cocktail Incubate with H R P- Conjugated Streptavidin Incubate with Detection Buffers Image with chemiluminescent imaging system Perform densitometry and analysis
Product Features	Easy to use No specialized equipment needed Compatible with nearly any liquid sample Proven technology (many publications) Highly sensitive (pg/ml) Sandwich ELISA specificity Higher density than ELISA, Western blot or bead-based multiplex
Storage/Stability	For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.

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AAH-CYT-1-4.pdf

AAH-CYT-1-4-datasheet.pdf

AAH-CYT-1-4-safety-datasheet.pdf