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Human Atherosclerosis Array C1

Human Atherosclerosis Array C1

ArtNr	AAH-ATH-1-8
Hersteller	Raybiotech
Menge	8 Sample Kit
Quantity options	2 Sample Kit 4 Sample Kit 8 Sample Kit
Kategorie	Oncology Arrays By Organ Colorectal Cancer Antibody Arrays
Typ	Array
Specific against	Human
Host	Hamster - Armenian
Citations	1. Woroniecki R P, Shatat I F, Supe K, Du Z F, Frederick J K.Urinary Cytokines and Steroid Responsiveness in Idiopathic Nephrotic Syndrome of Childhood. Am J Nephrol. 2008,28:83–90
ECLASS 10.1	32161000
ECLASS 11.0	32161000
UNSPSC	41116126
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Compatible Sample Types	Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates
Design Principle	Sandwich-based
Detection Method	Chemiluminescence
Features	Easy to use No specialized equipment needed Compatible with nearly any liquid sample Proven technology (many publications) Highly sensitive (pg/ml) Sandwich ELISA specificity Higher density than ELISA, Western blot or bead-based multiplex
Kit Components	Human Atherosclerosis Antibody Array C1 Membranes Blocking Buffer Wash Buffer 1 Wash Buffer 2 Biotinylated Detection Antibody Cocktail Streptavidin-Conjugated HRP Detection Buffer C Detection Buffer D Lysis Buffer 8-Well Incubation Tray Plastic Sheets Array Templates Manual
Number of Targets Detected	20
Other Materials Required	Pipettors, pipet tips and other common lab consumables Orbital shaker or oscillating rocker Tissue Paper, blotting paper or chromatography paper Adhesive tape or Saran Wrap Distilled or de-ionized water A chemiluminescent blot documentation system (such as UVPs ChemiDoc-It or EpiChem II Benchtop Darkroom), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline	Block membranes Incubate with Sample Incubate with Biotinylated Detection Antibody Cocktail Incubate with HRP-Conjugated Streptavidin Incubate with Detection Buffers Image with chemiluminescent imaging system Perform densitometry and analysis
Result Output	Semi-Quantitative
Short description	RayBio C-Series Human Atherosclerosis Antibody Array 1 Kit. Detects 20 Human Atherosclerotic Factors. Suitable for all liquid sample types.
Solid Support	Membrane
Storage	For best results, store the entire kit frozen at -20C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20C and all other reagents undiluted at 4C for no more than 3 months.
Targets Detected	CCL11, CCL2, CCL20, CD54, Eotaxin-1, G-CSF, GDNF, GM-CSF, ICAM-1, IFN-gamma, IL-1 alpha, IL-1 beta, IL-1 F1, IL-1 F2, IL-1 R1, IL-1 R2, M-CSF, MCP-1, MIP-3 alpha, PDGF-BB, RANTES, TGF beta 1, TGF beta 2, TGF beta 3, TNF alpha, TNF beta, TNFSF1B

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